Individual variation in the rate of oxygen consumption by zebrafish embryos
Individual variation in the rate of oxygen consumption by zebrafish embryos
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DOI：
10.1111/j.0022-1112.2004.00391.x
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发表时间：
2004-05-01
影响因子：
2
通讯作者：
Malte, H
中科院分区：
文献类型：
--
作者：
Bang, A;Gronkjær, P;Malte, H
采用基于微敏感传感器的方法，在受精后24 ~ 75 h，每隔6 h测量斑马鱼丹尼奥胚胎的耗氧量。在此期间，氧气消耗速率从4.54增加到8.29 nmol O(2)h（-1）。在个体水平上，耗氧量的差异导致受精后24 ~ 75 h的总耗氧量在0.261 ~ 0.462 μ mol O-2 /个体之间变化，平均为0.379 pmol O-2 /个体。一项单独的碳质量平衡研究证实了这一时期呼吸商为0.80的平均总耗氧量。这些结果表明，发育中的斑马鱼胚胎的代谢率存在显著的种内变异，这可能影响其他早期生活史性状，如生长和饥饿抗性。(C) 2004年不列颠群岛渔业协会。
A sensitive microsensor-based method was used to measure oxygen consumption of individual zebrafish Danio rerio embryos at 6 h intervals from 24 to 75 h post-fertilization. An increase in oxygen consumption rates from 4.54 to 8.29 nmol O(2)h(-1) was found during this period. At the individual level the differences in oxygen Consumption rates caused the total oxygen consumption from 24 to 75 h post-fertilization to vary between 0.261 and 0.462 mumol O-2 per individual with a mean of 0.379 pmol O-2 per individual. A separate carbon mass balance study corroborated the mean total oxygen consumption obtained by yielding a respiratory quotient of 0.80 for this period. These results suggest that there is significant intraspecific variation in the metabolic rate of developing zebrafish embryos, which may influence other early life-history traits such as growth and starvation resistance. (C) 2004 The Fisheries Society of the British Isles.