A novel water-soluble near-infrared fluorescent probe for monitoring mitochondrial viscosity.
A novel water-soluble near-infrared fluorescent probe for monitoring mitochondrial viscosity.
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DOI：
10.1016/j.talanta.2021.122592
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发表时间：
2021-10
期刊：
影响因子：
6.1
通讯作者：
Siqi Zhang;Yu Zhang;Lihe Zhao;Lanlan Xu;Hao Han;Yibing Huang;Q. Fei;Ying Sun;Pinyi Ma;D. Song
中科院分区：
文献类型：
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作者：
Siqi Zhang;Yu Zhang;Lihe Zhao;Lanlan Xu;Hao Han;Yibing Huang;Q. Fei;Ying Sun;Pinyi Ma;D. Song
线粒体是细胞能量的主要来源，在有氧呼吸过程中起着重要作用。一些刺激剂可导致线粒体微环境的改变，如粘度、pH和极性。线粒体粘度的异常变化与病理活动和疾病有关。因此，在不同的条件下关注线粒体粘度是至关重要的。通过两步反应合成了一种新型的水溶性有机分子JLQL。近红外传感器的最大发射波长为734.6 nm，斯托克斯位移为134.6 nm，由荧光团和作为受体基团的靶向分子组成，两者通过双键连接。随着PBS-甘油体系粘度的增加，传感器的荧光强度增加了175倍。可以减少其他微环境如pH和极性以及其他干扰分析物的干扰。JLQL能敏感地、选择性地区分莫能菌素和制霉菌素引起的不同水平的线粒体粘度。此外，探针可以提供一种有吸引力的方式来监测线粒体自噬期间粘度的实时变化。JLQL具有上述性质，可作为一种有效的线粒体粘度观测工具。
Mitochondria, the main source of energy of cells, play a significant role in aerobic respiration process. Some stimulants can result in changes of mitochondrial microenvironments such as viscosity, pH and polarity. Abnormal changes of mitochondrial viscosity have been shown to relate to pathological activities and diseases. Therefore, it is critical to focus our attention on mitochondrial viscosity under different conditions. A novel organic water-soluble molecule called JLQL that could monitor viscosity was conveniently synthesized in two steps. The near-infrared sensor with maximum emission wavelength of 734.6 nm and the Stokes shift of 134.6 nm consisted of a fluorophore and a mitochondrial-targeting moiety as an acceptor group; the two were connected by a double bond. The fluorescence intensity of the sensor increased 175 times with the enhancement of viscosity of a PBS-glycerol system. The interference of other microenvironments such as pH and polarity and other interference analytes could be reduced. JLQL could sensitively and selectively differentiate different levels of mitochondrial viscosity induced by monensin or nystatin. Furthermore, the probe may provide an attractive way to monitor real-time changes of viscosity during mitophagy. Possessing the above properties, JLQL can potentially be employed as a powerful tool for the observation of mitochondrial viscosity.