γ-Aminobutyric acid plays a key role in plant acclimation to a combination of high light and heat stress
γ-Aminobutyric acid plays a key role in plant acclimation to a combination of high light and heat stress
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DOI：
10.1093/plphys/kiac010
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发表时间：
2022-03-28
期刊：
影响因子：
7.4
通讯作者：
Zandalinas, Sara, I
中科院分区：
文献类型：
--
作者：
Balfagon, Damian;Gomez-Cadenas, Aurelio;Zandalinas, Sara, I
γ-氨基丁酸（gamma-aminobutyric acid，GABA）是植物适应强光和高温胁迫的重要物质，植物经常受到不同的非生物胁迫组合的影响，如强光（high light，HL）和高温。这些环境条件对农业生产构成威胁，影响光合作用，降低产量。植物的代谢反应，如碳水化合物和氨基酸通量的改变，在植物成功适应不同的非生物胁迫，引导资源对胁迫反应，抑制生长中起着关键作用。在这里，我们表明，拟南芥（拟南芥）植物HL或热胁迫（HS）的主要代谢反应是不同的植物受到HL和HS的组合（HL+HS）。我们进一步证明，在一个独特的代谢反应，包括糖和氨基酸的积累增加加上参与三羧酸循环的代谢产物的水平降低的组合应力的结果。在HL+HS期间专门积累的氨基酸中，我们鉴定了非蛋白质氨基酸γ-氨基丁酸（GABA）。对GABA生物合成缺陷的不同突变体（谷氨酸脱羧酶3 [gad 3]）以及自噬受损的突变体（自噬相关蛋白5和9 [atg 5和atg 9]）的分析表明，GABA在植物适应HL+HS中起关键作用，可能是通过促进自噬。综上所述，我们的研究结果确定了GABA在调节植物对复合胁迫的反应中的作用。
The amino acid gamma-aminobutyric acid orchestrates plant acclimation to a combination of high light and heat stress.Plants are frequently subjected to different combinations of abiotic stresses, such as high light (HL) intensity, and elevated temperatures. These environmental conditions pose a threat to agriculture production, affecting photosynthesis, and decreasing yield. Metabolic responses of plants, such as alterations in carbohydrates and amino acid fluxes, play a key role in the successful acclimation of plants to different abiotic stresses, directing resources toward stress responses, and suppressing growth. Here we show that the primary metabolic response of Arabidopsis (Arabidopsis thaliana) plants to HL or heat stress (HS) is different from that of plants subjected to a combination of HL and HS (HL+HS). We further demonstrate that the combined stress results in a unique metabolic response that includes increased accumulation of sugars and amino acids coupled with decreased levels of metabolites participating in the tricarboxylic acid cycle. Among the amino acids exclusively accumulated during HL+HS, we identified the nonproteinogenic amino acid gamma-aminobutyric acid (GABA). Analysis of different mutants deficient in GABA biosynthesis (GLUTAMATE DESCARBOXYLASE 3 [gad3]) as well as mutants impaired in autophagy (autophagy-related proteins 5 and 9 [atg5 and atg9]), revealed that GABA plays a key role in the acclimation of plants to HL+HS, potentially by promoting autophagy. Taken together, our findings identify a role for GABA in regulating plant responses to combined stress.