Differential Effects of Nicotine and Tobacco Smoke Condensate on Human Annulus Fibrosus Cell Metabolism
Differential Effects of Nicotine and Tobacco Smoke Condensate on Human Annulus Fibrosus Cell Metabolism
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DOI：
10.1002/jor.21417
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发表时间：
2011-10-01
影响因子：
2.8
通讯作者：
Kang, James
中科院分区：
文献类型：
--
作者：
Nam Vo;Wang, Dong;Kang, James
吸烟会增加椎间盘退变（IDD）和背部疼痛的风险，但吸烟不良影响的潜在机制在很大程度上尚不清楚。目前的假设预测，吸烟通过尼古丁介导的血管收缩间接导致缺乏症，这限制了椎间盘与周围环境之间的营养物质交换。我们假设椎间盘细胞，即位于最外层环的细胞和存在于退变椎间盘裂缝中的细胞，与含有可溶性烟草成分的血管系统直接接触，可能会扰乱正常的代谢活动，导致IDD。在这项研究中，我们通过比较直接暴露于烟草烟雾冷凝物和尼古丁对细胞活力和代谢活性的影响来验证我们的假设。我们发现烟冷凝物含有吸烟者吸入的所有水溶性化合物，比尼古丁对人体椎间盘细胞活力和代谢产生更大的有害影响。烟凝析液显著诱导炎症反应和金属蛋白酶基因表达，降低活性基质合成和基质结构基因表达。因此，我们已经证明，椎间盘细胞暴露于烟草烟雾成分有可能改变椎间盘基质稳态的负面后果。(C) 2011骨科研究学会，Wiley期刊出版公司[J]中华骨科杂志，2011
Tobacco smoking increases the risk of intervertebral disc degeneration (IDD) and back pain, but the mechanisms underlying the adverse effects of smoking are largely unknown. Current hypotheses predict that smoking contributes to IDD indirectly through nicotine-mediated vasoconstriction which limits the exchange of nutrients between the discs and their surroundings. We alternatively hypothesize that direct contact of disc cells, that is, cells in the outermost annulus and those present along fissures in degenerating discs, with the vascular system containing soluble tobacco smoking constituents could perturb normal metabolic activities resulting in IDD. In this study, we tested our hypothesis by comparing the effects of direct exposure of human disc cells to tobacco smoke condensate and nicotine on cell viability and metabolic activity. We showed that smoke condensate, which contains all of the water-soluble compounds inhaled by smokers, exerts greater detrimental effects on human disc cell viability and metabolism than nicotine. Smoke condensate greatly induced an inflammatory response and gene expression of metalloproteinases while reduced active matrix synthesis and expression of matrix structural genes. Therefore, we have demonstrated that disc cell exposure to the constituents of tobacco smoke has negative consequences which have the potential to alter disc matrix homeostasis. (C) 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:1585-1591, 2011