APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
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DOI：
10.1038/ncb2347
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发表时间：
2011-09-18
影响因子：
21.3
通讯作者：
中科院分区：
文献类型：
--
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有丝分裂过程中的忠实染色体隔离取决于纺锤体组装检查点（SAC），可监视动力学螺旋的固定膜。最后的动物学固定在主轴上对了解细胞如何保持基因组稳定性。当囊满足时，我们包括APC15介导了APC/C上CDC20和MCC的恒定周转率，以使SAC响应动力学的附着状态。
Faithful chromosome segregation during mitosis depends on the Spindle Assembly Checkpoint (SAC) that monitors kinetochore attachment to the mitotic spindle. Unattached kinetochores generate mitotic checkpoint proteins complexes (MCCs) that bind and inhibit the Anaphase Promoting Complex/Cyclosome (APC/C). How the SAC proficiently inhibits the APC/C but still allows its rapid activation when the last kinetochore attaches to the spindle is important to understand how cells maintain genomic stability. We show that the APC/C subunit APC15 is required for the turnover of the APC/C co-activator Cdc20 and release of MCCs during SAC signalling but not for APC/C activity per se. In the absence of APC15, MCCs and ubiquitylated Cdc20 remain ‘locked’ onto the APC/C, which prevents the ubiquitylation and degradation of Cyclin B1 when the SAC is satisfied. We conclude that APC15 mediates the constant turnover of Cdc20 and MCCs on the APC/C to allow the SAC to respond to the attachment state of kinetochores.
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影响因子：
21.3
作者：
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Pines, Jonathon
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