Lower proportion of CD45R0+ cells and deficient interleukin-10 production by formula-fed infants, compared with human-fed, is corrected with supplementation of long-chain polyunsaturated fatty acids
Lower proportion of CD45R0+ cells and deficient interleukin-10 production by formula-fed infants, compared with human-fed, is corrected with supplementation of long-chain polyunsaturated fatty acids
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DOI：
10.1097/00005176-200009000-00017
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发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Clandinin, MT
中科院分区：
文献类型：
--
作者：
Field, CJ;Thomson, CA;Clandinin, MT
背景资料：在早产儿配方奶粉中添加20：4 n-6和22：6 n-3脂肪酸的免疫后果尚不清楚。方法：测定喂养早产儿（14-42天）人乳（人乳组）、婴儿配方奶粉组或添加长链多不饱和脂肪酸20：4 n-6和22：6 n-3的配方奶粉（配方奶粉+ LCP组）对分离的外周血淋巴细胞（通过流式细胞术）和脂质组成（通过气液色谱法）的影响。结果：随着年龄的增长，母乳组和配方奶粉+ LCP组的CD 3（+）CD 4（+）T细胞百分比和CD 20（+）T细胞百分比均呈上升趋势（P < 0.05），而未添加配方奶粉组则无此趋势。与配方奶粉组相比，配方奶粉+ LCP组和人乳组的CD 4（+）细胞在42日龄时表达更多的CD 45 R 0（抗原成熟）和更少的CD 45 RA（抗原初始）（P < 0.05）。在42天时，配方物组的细胞中IL-10产生低于人乳组的细胞（P < 0.05）。配方+ LCP组细胞产生的IL-10与人乳组细胞产生的IL-10没有差异。观察到配方奶粉+ LCP淋巴细胞产生的sIL-2 R与年龄相关的降低（P < 0.05），但配方奶粉+ LCP组42天时的sIL-2 R产生与人乳组无显著差异。结论：添加LCP的早产儿配方奶粉中淋巴细胞数量、磷脂组成、细胞因子产生和抗原成熟度与母乳喂养的早产儿配方奶粉中比较，差异有统计学意义（P <0. 05）。这可能会影响婴儿对免疫挑战的反应能力。
Background: The immune consequences of adding 20:4n-6 and 22:6n-3 fatty acids to preterm infant formula are not known.Methods: The effect of feeding preterm infants (14-42 days of age) human milk (Human Milk group), infant formula formula group), or formula with added long-chain polyunsaturated fatty acids 20:4n-6 and 22:6n-3 (Formula + LCP group) on isolated peripheral blood lymphocytes (by flow cytometry) and lipid composition (by gas-liquid chromatography) was determined. Lymphocytes were stimulated in vitro with phytohemagglutinin to measure soluble interleukin (sIL)-2R and IL-10 production (by enzyme-linked immunosorbent assay).Results: With age, the percentage of CD3(+)CD4(+) T cells and the percentage of CD20(+) cells increased in the Human Milk and Formula + LCP groups (P < 0.05), but not in the unsupplemented Formula group. Compared with the Formula group, CD4(+) cells from the Formula + LCP and Human Milk groups expressed more CD45R0 (antigen mature) and less CD45RA (antigen naive) at 42 days of age (P < 0.05). At 42 days, IL-10 production was lower (P < 0.05) in cells of the Formula group than in cells of the Human Milk group. Production of IL-10 by the cells of the Formula + LCP group was not different from that produced by the Human Milk group cells. An age-related decrease (P < 0.05) in sIL-2R production by Formula + LCP lymphocytes was observed, but sIL-2R production at 42 days in the Formula + LCP group did not differ significantly from that in the Human Milk group. Compared with Formula alone, adding LCP to formula resulted in a lower C18:2n-6 and higher C20:4n-6 content in lymphocyte phospholipids (P < 0.05).Conclusions: Adding LCP to a preterm infant formula resulted in lymphocyte populations, phospholipid composition, cytokine production, and antigen maturity that are more consistent with that in human milk-fed infants. This may affect the ability of the infant to respond to immune challenges.