Sequence analysis of L RNA of Lassa virus
Sequence analysis of L RNA of Lassa virus
复制标题
DOI：
10.1016/j.virol.2003.09.009
复制
发表时间：
2004-01-05
期刊：
影响因子：
3.7
通讯作者：
Günther, S
中科院分区：
文献类型：
--
作者：
Vieth, S;Torda, AE;Günther, S
对来源于尼日利亚、加纳/象牙海岸和塞拉利昂的3株拉沙病毒的L RNA进行了测序，并对数据进行了结构预测和系统发育分析。L基因产物长度为2218-2221个氨基酸残基，氨基酸序列差异达18%，并含有多个保守区。只有一个504个残基的区域（位置1043-1546）可以被分配功能，即RNA聚合酶的功能。二级结构的预测表明，这一领域是非常相似的RNA依赖的RNA聚合酶的已知结构编码的正链RNA病毒，允许建立一个模型。在聚合酶区域之外，除了具有强α-螺旋内容的区域和可能在N末端的卷曲螺旋结构域之外，几乎没有结构数据。没有证据表明在拉沙病毒进化过程中发生了重配或重组，RNA聚合酶区的二级结构辅助比对允许可靠地重建所有负链RNA病毒的同源性，表明沙粒病毒科与Nairoviritses病毒的亲缘关系最近。总之，这些数据为拉沙病毒L蛋白的结构和功能表征提供了基础，并揭示了对负链RNA病毒复制的新见解。(C)2003年爱思唯尔公司All rights reserved.
The L RNA of three Lassa virus strains originating from Nigeria, Ghana/Ivory Coast, and Sierra Leone was sequenced and the data subjected to structure predictions and phylogenetic analyses. The L gene products had 2218-2221 residues, diverged by 18% at the amino acid level, and contained several conserved regions. Only one region of 504 residues (positions 1043-1546) could be assigned a function, namely that of an RNA polymerase. Secondary structure predictions suggest that this domain is very similar to RNA-dependent RNA polymerases of known structure encoded by plus-strand RNA viruses, permitting a model to be built. Outside the polymerase region, there is little structural data, except for regions of strong alpha-helical content and probably a coiled-coil domain at the N terminus. No evidence for reassortment or recombination during Lassa virus evolution was found. The secondary structure-assisted alignment of the RNA polymerase region permitted a reliable reconstruction of the phylogeny of all negative-strand RNA viruses, indicating that Arenaviridae are most closely related to Nairoviritses. In conclusion, the data provide a basis for structural and functional characterization of the Lassa virus L protein and reveal new insights into the phylogeny of negative-strand RNA viruses. (C) 2003 Elsevier Inc. All rights reserved.