Polyethylene glycol influences microbial interactions with intestinal epithelium.
Polyethylene glycol influences microbial interactions with intestinal epithelium.
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聚乙二醇影响微生物与肠上皮的相互作用。
DOI：
10.1097/shk.0b013e31818348a5
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Wells,CarolL
中科院分区：
文献类型：
--
作者：
Henry-Stanley,MichelleJ;Wells,CarolL
有新的证据表明，广泛用作术前肠道准备的聚乙二醇（PEG）可保护肠上皮免受微生物侵袭。设计实验以研究低分子量（LMW; 3.35 kd）和高分子量（HMW; 15-20 kd）PEG对大肠杆菌、白色念珠菌和光滑念珠菌与肠上皮细胞相互作用的影响（这三种肠道微生物经常参与休克和创伤患者的全身感染）。体外实验研究了PEG对成熟Caco-2肠上皮细胞的影响。采用庆大霉素保护试验，HMW和LMW PEG均抑制E. Caco-2肠上皮细胞对大肠杆菌的内化作用。用免疫吸附试验，HMW和LMW PEG都抑制C.白色念珠菌和C.光滑粘附于Caco-2肠上皮细胞。在HMW或LMW PEG中孵育的Caco-2细胞的扫描电子显微镜照片显示，球状物质随机分布在上皮表面，并且顶端微绒毛似乎扭曲。作为与这些实验体内相关性，将抗生素处理的小鼠的不同组与E. coli、C.白色念珠菌或C.光滑组和队列组给予含有5%HMW或5%LMW PEG的饮用水。盲肠定植E.在给予HMW但不给予LMW PEG的小鼠中，大肠杆菌的表达减少。盲肠定植C. albicans)和非白念珠菌(C.在给予HMW或LMW PEG的小鼠中，光滑脂肪减少。这些数据提供了进一步的证据，表明PEG可减少微生物定植和微生物与肠上皮的相互作用。
There is emerging evidence that polyethylene glycol (PEG), widely used as a bowel preparation before surgery, may protect the intestinal epithelium from microbial invasion. Experiments were designed to study the effects of both low-molecular-weight (LMW; 3.35 kd) and high-molecular-weight (HMW; 15-20 kd) PEG on interactions of Escherichia coli, Candida albicans, and Candida glabrata with intestinal epithelium (these three intestinal microbes are frequently involved in systemic infection in shock and trauma patients.) In vitro experiments studied the effects of PEG on mature Caco-2 enterocytes. Using the gentamicin protection assay, both HMW and LMW PEG inhibited E. coli internalization by Caco-2 enterocytes. Using an immunosorbent assay, both HMW and LMW PEG inhibited C. albicans and C. glabrata adherence to Caco-2 enterocytes. Scanning electron micrographs of Caco-2 cells incubated in HMW or LMW PEG showed globular material distributed randomly over the epithelial surface, and apical microvilli seemed distorted. As an in vivo correlate to these experiments, separate groups of antibiotic-treated mice were orally associated with either E. coli, C. albicans, or C. glabrata, and cohort groups were given drinking water containing 5% HMW or 5% LMW PEG. Cecal colonization of E. coli was decreased in mice given HMW but not LMW PEG. Cecal colonization with C. albicans or C. glabrata was decreased in mice given either HMW or LMW PEG. These data provide further evidence that PEG may decrease microbial colonization and microbial interactions with intestinal epithelium.