Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging.
Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging.
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DOI：
10.1251/bpo22
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发表时间：
2001-12-11
影响因子：
6.4
通讯作者：
Wold LE
中科院分区：
文献类型：
--
作者：
Ren J;Wold LE
20多年来，分离的成人心室肌细胞一直是心血管研究的有用模型。随着细胞生理学和转基因技术的发展，直接测量离体心室肌细胞力学正成为心脏生理学中越来越重要的技术，它提供了心脏兴奋-收缩偶联的基础信息，无论是在药物干预还是病理状态下。本文的目的是描述从大鼠和小鼠的心室肌细胞的分离，并使用实时节拍到节拍同时记录心肌细胞收缩和细胞内Ca 2+瞬变。
Isolated adult cardiac ventricular myocytes have been a useful model for cardiovascular research for more than 20 years. With the recent advances in cellular physiology and transgenic techniques, direct measurement of isolated ventricular myocyte mechanics is becoming an increasingly important technique in cardiac physiology that provides fundamental information on excitation-contraction coupling of the heart, either in drug intervention or pathological states. The goal of this article is to describe the isolation of ventricular myocytes from both rats and mice, and the use of real-time beat-to-beat simultaneous recording of both myocyte contraction and intracellular Ca2+ transient.