Limits of Peripheral Blood Mononuclear Cells for Gene Expression-Based Biomarkers in Juvenile Idiopathic Arthritis.
Limits of Peripheral Blood Mononuclear Cells for Gene Expression-Based Biomarkers in Juvenile Idiopathic Arthritis.
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DOI：
10.1038/srep29477
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发表时间：
2016-07-07
影响因子：
4.6
通讯作者：
Jarvis JN
中科院分区：
文献类型：
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作者：
Wong L;Jiang K;Chen Y;Hennon T;Holmes L;Wallace CA;Jarvis JN
青少年特发性关节炎(JIA)是影响美国儿童的最常见的慢性疾病之一。与许多风湿性疾病一样，人们对利用基因组技术开发用于诊断或指导治疗的生物标记物(“个性化药物”)的兴趣日益浓厚。在这里，我们探索使用外周血单个核细胞(PBMC)中的基因表达模式作为开发此类生物标记物的第一步方法。尽管PBMC在翻译研究中具有许多理论优势，但我们发现，样本的异质性使得PBMC上的RNAseq不适合作为筛选JIA候选生物标记物的第一步方法。对同质细胞群体的RNAseq研究更有可能是有用的和信息丰富的。
Juvenile Idiopathic Arthritis (JIA) is one of the most common chronic disease conditions affecting children in the USA. As with many rheumatic diseases, there is growing interest in using genomic technologies to develop biomarkers for either diagnosis or to guide treatment (“personalized medicine”). Here, we explore the use of gene expression patterns in peripheral blood mononuclear cells (PBMC) as a first step approach to developing such biomarkers. Although PBMC carry many theoretical advantages for translational research, we have found that sample heterogeneity makes RNASeq on PBMC unsuitable as a first-step method for screening biomarker candidates in JIA. RNASeq studies of homogeneous cell populations are more likely to be useful and informative.