Cell-sized lipid vesicles for cell-cell synaptic therapies.
Cell-sized lipid vesicles for cell-cell synaptic therapies.
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用于细胞间突触治疗的细胞大小的脂质囊泡。
DOI：
10.1142/s233954781750011x
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Lee,AP
中科院分区：
文献类型：
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作者：
Vallejo,D;Lee,SH;Lee,D;Zhang,C;Rapier,C;Chessler,SD;Lee,AP
细胞大小的脂质囊泡（CLV）在治疗和人工细胞应用方面显示出巨大的前景，但它们的脆弱性和短保质期阻碍了广泛采用和商业可行性。我们提出了一种方法来规避CLV的存储限制，如巨大的单层囊泡（GUV）和单室多体（SCM），通过将它们存储在一个双重乳液前体的形式。双重乳液可储存至少8个月，并随时容易地转化为GUV或SCM。在这项研究中，我们调查了负责这种形态变化的界面参数，我们还证明了CLVs的治疗潜力，利用它们提出一个跨膜蛋白，神经连接素-2，胰腺细胞，形成细胞-细胞突触，刺激胰岛素分泌和细胞生长。
Cell-sized lipid vesicles (CLVs) have shown great promise for therapeutic and artificial cell applications, but their fragility and short shelf life has hindered widespread adoption and commercial viability. We present a method to circumvent the storage limitations of CLVs such as giant unilamellar vesicles (GUVs) and single-compartment multisomes (SCMs) by storing them in a double emulsion precursor form. The double emulsions can be stored for at least 8 months and readily converted into either GUVs or SCMs at any time. In this study, we investigate the interfacial parameters responsible for this morphological change, and we also demonstrate the therapeutic potential of CLVs by utilizing them to present a transmembrane protein, neuroligin-2, to pancreatic-cells, forming cell-cell synapses that stimulate insulin secretion and cellular growth.