Vaccinia virus A43R gene encodes an orthopoxvirus-specific late non-virion type-1 membrane protein that is dispensable for replication but enhances intradermal lesion formation.
Vaccinia virus A43R gene encodes an orthopoxvirus-specific late non-virion type-1 membrane protein that is dispensable for replication but enhances intradermal lesion formation.
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痘苗病毒 A43R 基因编码正痘病毒特异性晚期非病毒体 1 型膜蛋白，该蛋白对于复制来说是必需的，但会增强皮内病变的形成。
DOI：
10.1016/j.virol.2009.10.025
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发表时间：
2010
期刊：
影响因子：
3.7
通讯作者：
Moss,Bernard
中科院分区：
文献类型：
--
作者：
Sood,CindyL;Moss,Bernard
牛痘病毒A43 R开放阅读框编码168个氨基酸的蛋白质，具有预测的N-末端信号序列和C-末端跨膜结构域。尽管A43 R在正痘病毒属的所有测序成员中是保守的，但没有识别出非正痘病毒同源物或功能基序。生化和共聚焦显微镜研究表明，A43在病毒DNA合成后的晚期表达，是一种具有两个N-连接寡糖链的1型膜蛋白。A43存在于高尔基体和质膜中，但在蔗糖梯度纯化的成熟病毒体中仅检测到痕量，而在CsCl梯度纯化的包膜病毒体中未检测到。预防A43 R表达对空斑大小或细胞培养中的病毒复制没有影响，对小鼠鼻内感染后的毒力影响不大。虽然A43突变体在小鼠皮肤上产生的病变明显小于对照组，但从病变中回收的病毒量相似。
The vaccinia virus A43R open reading frame encodes a 168-amino acid protein with a predicted N-terminal signal sequence and a C-terminal transmembrane domain. Although A43R is conserved in all sequenced members of the orthopoxvirus genus, no non-orthopoxvirus homolog or functional motif was recognized. Biochemical and confocal microscopic studies indicated that A43 is expressed at late times following viral DNA synthesis and is a type-1 membrane protein with two N-linked oligosaccharide chains. A43 was present in Golgi and plasma membranes but only a trace amount was detected in sucrose gradient purified mature virions and none in CsCl gradient purified enveloped virions. Prevention of A43R expression had no effect on plaque size or virus replication in cell culture and little effect on virulence after mouse intranasal infection. Although the A43 mutant produced significantly smaller lesions in skin of mice than the control, the amounts of virus recovered from the lesions were similar.