Age-related changes in glutathione availability and skeletal muscle carbonyl content in healthy rats
Age-related changes in glutathione availability and skeletal muscle carbonyl content in healthy rats
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DOI：
10.1016/j.exger.2003.10.014
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发表时间：
2004-02-01
影响因子：
3.9
通讯作者：
Mirand, PP
中科院分区：
文献类型：
--
作者：
Mosoni, L;Breuillé, D;Mirand, PP
衰老的自由基理论认为，氧化应激在衰老过程中起着关键作用。通过改变肌肉蛋白质降解速率，它可以加速与年龄相关的肌肉蛋白质损失。谷胱甘肽（GSH）是机体主要的抗氧化剂之一，可防止这一现象。但其浓度在老化过程中降低。我们的目的是更好地了解与年龄相关的谷胱甘肽可用性降低的机制以及与肌肉减少症的联系。选用6、9、12、15、19、22、25和28月龄Wistar大鼠（n = 6/月龄），测定血浆和骨骼肌蛋白羰基含量、血浆总胱氨酸和游离胱氨酸含量、肝脏和肌肉谷胱甘肽含量以及肝脏GSSG还原酶、谷胱甘肽过氧化物酶、谷胱甘肽转移酶和γ谷氨酰半胱氨酸合成酶（GCS）活性。虽然组织谷胱甘肽含量随着年龄的增长而下降，但氧化应激的其他标志物在衰老过程中几乎没有变化。尤其是。肌肉蛋白质羰基含量无变化。谷胱甘肽利用率的变化不能用半胱氨酸的利用率来解释，而取决于γ GCS活性。骨骼肌羰基含量在衰老过程中的稳定性表明肌肉中氧化蛋白质的降解非常有效。(C)2003年爱思唯尔公司所有的战斗保留。
The free radical theory of aging proposes that oxidative stress plays a key role in the aging process. By altering muscle protein degradation rates, it could accelerate the age-related loss of muscle proteins. Glutathione (GSH), one of the main body antioxidants, could prevent this phenomenon. but its concentration decreases during aging. Our aims were to have a better understanding of the mechanisms of the age-related decrease in glutathione availability and of the links with sarcopenia. Mate Wistar rats aged 6, 9, 12, 15, 19, 22, 25 and 28 months (n = 6 per age) were used to measure plasma and skeletal muscle protein carbonyl content, plasma total and free cyst(e)ine content, liver and muscle glutathione content as well as liver GSSG reductase, GSH peroxidase, GSH transferase and gamma glutamyl cysteine synthetase (GCS) activities. Although tissue glutathione content decreased with age, the other markers of oxidative stress were little changed during aging. In particular. muscle protein carbonyl content was unchanged. Variations in glutathione availability were not explained by cyst(e)ine availability but depended on gamma GCS activity. The stability of skeletal muscle carbonyl content during aging suggests a very efficient degradation of oxidized proteins in muscle. (C) 2003 Elsevier Inc. All fights reserved.