Impact of chloroplastic- and extracellular-sourced ROS on high light-responsive gene expression in Arabidopsis
Impact of chloroplastic- and extracellular-sourced ROS on high light-responsive gene expression in Arabidopsis
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DOI：
10.1093/jxb/erm289
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发表时间：
2008-02-01
影响因子：
6.9
通讯作者：
Mullineaux, Philip M.
中科院分区：
文献类型：
--
作者：
Bechtold, Ulrike;Richard, Odile;Mullineaux, Philip M.
分析了拟南芥28个强光反应基因APX2在环境和生理因子作用下的表达情况。大多数(81%)HL反应基因，包括APX2，它们的表达需要光合作用的电子传递，并且对脱落酸(ABA；68%)有反应，这强化了这两个信号在HL反应基因表达中至关重要的印象。此外，通过使用在ROS代谢中改变的突变体，包括APX2在内的这些基因中的61%可能对叶绿体来源的ROS做出响应。相反，质外体/质膜来源的过氧化氢，部分由呼吸爆发NADPH氧化酶AtrbohD和AtrbohF控制，被证明只对APX2的表达重要。APX2在叶片中的表达仅限于束鞘组织；然而，对于本研究中的其他基因，关于其组织特异性表达的信息很少。与远端叶片相比，在HL和对照叶片中，叶柄中富含束鞘组织的基因表达分析表明，其中25%的基因在叶柄中的表达是叶片中的10倍。然而，这并不意味着这些叶柄表达基因遵循观察到的APX2的调节模式。
The expression of 28 high light (HL)-responsive genes of Arabidopsis was analysed in response to environmental and physiological factors known to influence the expression of the HL-responsive gene, ASCORBATE PEROXIDASE2 (APX2). Most (81%) of the HL-responsive genes, including APX2, required photosynthetic electron transport for their expression, and were responsive to abscisic acid (ABA; 68%), strengthening the impression that these two signals are crucial in the expression of HL-responsive genes. Further, from the use of mutants altered in reactive oxygen species (ROS) metabolism, it was shown that 61% of these genes, including APX2, may be responsive to chloroplast-sourced ROS. In contrast, apoplastic/plasma membrane-sourced H2O2, in part directed by the respiratory burst NADPH oxidases AtrbohD and AtrbohF, was shown to be important only for APX2 expression. APX2 expression in leaves is limited to bundle sheath parenchyma; however, for the other genes in this study, information on their tissue specificity of expression is sparse. An analysis of expression in petioles, enriched for bundle sheath tissue compared with distal leaf blade, in HL and control leaves showed that 25% of them had > 10-fold higher expression in the petiole than in the leaf blade. However, this did not mean that these petiole expression genes followed a pattern of regulation observed for APX2.