Identification, expression and tissue distribution of a renalase homologue from mouse
Identification, expression and tissue distribution of a renalase homologue from mouse
复制标题
小鼠肾酶同源物的鉴定、表达和组织分布
DOI：
10.1007/s11033-007-9131-1
复制
发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Zhang, Shu-Ping
中科院分区：
文献类型：
--
作者：
Wang, Jian;Qi, Shaoling;Zhang, Shu-Ping
黄素腺嘌呤二核苷酸依赖的单胺氧化酶在许多生物过程中起着重要的作用。肾酶是近年来在人类肾脏中发现的一种新的单胺氧化酶，在终末期肾病（ESRD）患者中表达明显降低。在这里，我们报告了从小鼠肾酶同源物的鉴定，称为mMAO-C（小鼠单胺氧化酶-C）后的单胺氧化酶-A和-B（MAO-A和-B）。该基因位于小鼠19 C1染色体上，编码区跨越7个外显子。推测其氨基酸序列含有一个典型的分泌信号肽和一个保守的胺氧化酶结构域。系统发育分析和多序列比对结果表明，所有物种中均存在mMAO-C样序列，且具有显著的相似性。该基因已提交至NCBI GenBank数据库（登录号：DQ 788834）。利用克隆的mMAO-C基因构建表达载体，在原核和真核细胞中均能有效表达外源蛋白。重组mMAO-C蛋白在人细胞株中得到分泌表达，表明其信号肽具有生物学功能。组织表达谱分析显示，mMAO-C基因主要在小鼠肾脏和睾丸中表达，提示肾脏和睾丸是肾酶分泌的主要来源。总之，这项研究为了解mMAO-C及其同系物在内分泌中的生理和生物学功能提供了一个深入的了解。
FAD (flavin adenine dinucleotide)-dependent monoamine oxidases play very important roles in many biological processes. A novel monoamine oxidase, named renalase, has been identified in human kidney recently and is found to be markedly reduced in patients with end-stage renal disease (ESRD). Here, we reported the identification of a renalase homologue from mouse, termed mMAO-C (mouse monoamine oxidase-C) after the monoamine oxidase-A and -B (MAO-A and -B). This gene locates on the mouse chromosome 19C1 and its coding region spans 7 exons. The deuced amino acid sequences were predicted to contain a typical secretive signal peptide and a conserved amine oxidase domain. Phylogenetic analysis and multiple sequences alignment indicated that mMAO-C-like sequences exist in all examined species and share significant similarities. This gene has been submitted to the NCBI GenBank database (Accession number: DQ788834). With expression vectors generated from the cloned mMAO-C gene, exogenous protein was effectively expressed in both prokaryotic and eukaryotic cells. Recombinant mMAO-C protein was secreted out of human cell lines, indicating the biological function of its signal peptide. Moreover, tissue expression pattern analysis revealed that mMAO-C gene is predominantly expressed in the mouse kidney and testicle, which implies that kidney and testicle are the main sources of renalase secretion. Shortly, this study provides an insight into understanding the physiological and biological functions of mMAO-C and its homologues in endocrine.