Visual histological grading system for the evaluation of in vitro-generated neocartilage
Visual histological grading system for the evaluation of in vitro-generated neocartilage
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DOI：
10.1089/ten.2006.12.2141
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发表时间：
2006-08-01
影响因子：
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通讯作者：
Mainil-Varlet, Pierre
中科院分区：
文献类型：
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作者：
Grogan, Shawn Patrick;Barbero, Andrea;Mainil-Varlet, Pierre
在这里，我们提出了一种可视化评估系统的发展，用于体外工程软骨组织的常规评估。通过在颗粒培养系统或无支架生物反应器系统中培养人关节软骨细胞来产生新的软骨细胞。所有组织均经石蜡包埋，石蜡切片，藏红O-固绿染色。每个样本的评价分为3类(藏红花O染色的均匀性和强度、细胞间距/产生的基质数量和细胞形态)，每类包括4个组成部分，得分从0到3。3名观察员对每个样本进行评估，并与客观的计算机组织形态计量学系统进行独立测试。还对微丸进行了糖胺聚糖(GAG)含量的生化评估。小球组织学评分与GAG含量显著相关，并与计算机组织形态计量学系统一致。该系统允许对体外产生的软骨组织进行有效和快速的评估，该评估与指示软骨质量的客观参数相关。
Here we present the development of a visual evaluation system for routine assessment of in vitro-engineered cartilaginous tissue. Neocartilage was produced by culturing human articular chondrocytes in pellet culture systems or in a scaffold-free bioreactor system. All engineered tissues were embedded in paraffin and were sectioned and stained with Safranin O-fast green. The evaluation of each sample was broken into 3 categories (uniformity and intensity of Safranin O stain, distance between cells/amount of matrix produced, and cell morphology), and each category had 4 components with a score ranging from 0 to 3. Three observers evaluated each sample, and the new system was independently tested against an objective computer-based histomorphometry system. Pellets were also assessed biochemically for glycosaminoglycan (GAG) content. Pellet histology scores correlated significantly with GAG contents and were in agreement with the computer-based histomorphometry system. This system allows a valid and rapid assessment of in vitro-generated cartilaginous tissue that has a relevant association with objective parameters indicative of cartilage quality.