Imaging transcription elongation dynamics by new technologies unveils the organization of initiation and elongation in transcription factories
Imaging transcription elongation dynamics by new technologies unveils the organization of initiation and elongation in transcription factories
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通过新技术对转录延伸动态进行成像揭示了转录工厂中起始和延伸的组织
DOI：
10.1016/j.ceb.2022.01.002
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发表时间：
2022
影响因子：
7.5
通讯作者：
Sato Yuko
中科院分区：
文献类型：
--
作者：
Kimura Hiroshi;Sato Yuko
在哺乳动物细胞中，RNA聚合酶II(RNAP2)介导的转录发生在细胞核中的许多灶中。由RNAP2活性检测到的转录灶通常被称为转录工厂。最近的超分辨显微镜技术揭示了RNAP2在活细胞中的聚集和焦点形成，这取决于其C末端结构域(CTD)中重复序列的磷酸化状态。使用来自磷酸化特异性抗体的活细胞探针也跟踪了RNAP2的差异磷酸化起始和进行性延长形式。最近的活细胞观察检测到空间上分离的RNAP2起始和延伸焦点，表明存在转录起始和延伸工厂。
In mammalian cells, RNA polymerase II (RNAP2)-mediated transcription occurs in numerous foci in the nucleus. Transcription foci detected by RNAP2 activity have often been called transcription “factories”. Recent super-resolution microscopy techniques have revealed clustering and focus formation of RNAP2 in living cells, depending on phosphorylation states of repeats in its C-terminal domain (CTD). The differentially phosphorylated initiation and processive elongation forms of RNAP2 have also been tracked using live-cell probes derived from phosphorylation-specific antibodies. Recent live cell observations have detected spatially separated initiation and elongating RNAP2 foci, suggesting the presence of transcription initiation and elongation factories.