Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection
Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection
复制标题
葡萄糖氧化酶指导的细胞内葡萄糖检测荧光放大策略
DOI：
10.1021/acsami.9b00010
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发表时间：
2019-03-20
影响因子：
9.5
通讯作者：
Lin, Jing
中科院分区：
文献类型：
--
作者：
Jiang, Shanshan;Zhang, Yifan;Lin, Jing
The accurate detection of glucose at cellular level remains a big challenge. In this study, a signal amplification strategy mediated by silver nanocube (AgNC), glucose oxidase (GOx), and silver ion fluorescence probe (denoted as AgNC Gox/Ag+-FP) is proposed for amplified intracellular glucose detection. The AgNC is oxidized into Ag+ by H2O2 generated from GOx-catalyzed glucose oxidation reaction, and Ag+ remarkably enhances the red fluorescence of Ag+-FP. Our results show that AgNC Gox/Ag+-FP is highly sensitive and specific to glucose and H2O2. Afterward, the feasibility of using AgNC Gox/Ag+-FP to detect intracellular glucose is verified in five different cell lines. In summary, a sensitive and specific fluorescence amplification strategy has been developed for intracellular glucose detection.
