Novel highly hydrophilic methacrylate-based monolithic column with mixed-mode of hydrophilic and strong cation-exchange interactions for pressurized capillary electrochromatography
Novel highly hydrophilic methacrylate-based monolithic column with mixed-mode of hydrophilic and strong cation-exchange interactions for pressurized capillary electrochromatography
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用于加压毛细管电色谱的新型高亲水性甲基丙烯酸酯整体柱，具有亲水性和强阳离子交换相互作用的混合模式
DOI：
10.1016/j.chroma.2011.05.052
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发表时间：
2011-07-22
影响因子：
4.1
通讯作者：
Xie, Zenghong
中科院分区：
文献类型：
--
作者：
Lin, Jia;Liu, Shaofeng;Xie, Zenghong
基于2-丙烯酰胺基-2-甲基-1-丙磺酸(AMPS)和季戊四醇三丙烯酸酯(PETA)的共聚反应，设计了一种新型的加压毛细管电泳色谱高亲水性聚甲基丙烯酸酯整体固定相。当流动相中乙腈(ACN)含量超过25%时，可观察到典型的亲水相互作用机理。渗透性研究中观察到不同极性流动相的轻微膨胀或收缩。在亲水作用层析模式下，流动相中ACN的含量仅为50%左右，对中性酰胺和苯酚等极性分析物有很好的保留和高效分离。这明显低于之前报道的亲水性聚甲基丙烯酸酯单体中使用的ACN(>；90%)的含量。此外，在带电多肽的分析中，还可以得到亲水相互作用(HI)和强阳离子交换(SCX)的混合模式，柱效高达8万盘/m，没有峰拖尾。所制备的亲水性固定相消耗的有机溶剂量少，可能为极性溶质提供一种潜在的环境友好型分离介质。(C)2011爱思唯尔B.V.保留所有权利。
A novel highly hydrophilic polymethacrylate-based monolithic stationary phase based on the copolymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and pentaerythritol triacrylate (PETA) was designed for pressurized capillary electrochromatography. A typical hydrophilic interaction chromatography mechanism could be observed when the content of acetonitrile (ACN) in the mobile phase exceeded 25%. Slight swelling or shrinking with mobile phases of different polarity was observed in permeability studies. Good retentions and efficient separations of polar analytes, such as neutral amides and phenols, were well achieved in hydrophilic interaction chromatography mode with only about 50% ACN content in the mobile phase. It was remarkably lower than the content of ACN (>90%) used on the hydrophilic polymethacrylate-based monoliths reported previously. Additionally, a mixed mode of hydrophilic interaction (HI) and strong cation-exchange (SCX) could be also obtained in the analysis of charged peptides, and high column efficiency up to 80,000 plates/m was achieved without peak tailing. The prepared hydrophilic stationary phase might provide a potential environmental friendly separation media for polar solutes as it consumes a low volume of organic solvents. (C) 2011 Elsevier B.V. All rights reserved.