Osteogenic effects of bioabsorbable magnesium implant in rat mandibles and in vitro
Osteogenic effects of bioabsorbable magnesium implant in rat mandibles and in vitro
复制标题
生物可吸收镁植入物对大鼠下颌骨和体外的成骨作用
DOI：
10.1002/jper.20-0162
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发表时间：
2020-09-10
影响因子：
4.3
通讯作者：
Qiu, Jiaxuan
中科院分区：
文献类型：
--
作者：
He, Wei;Zhang, Hai;Qiu, Jiaxuan
背景骨增量或骨移植通常需要用于牙种植体的放置或骨缺损的外科重建。生物可吸收镁植入物显示出促进长骨中的成骨。本研究的目的是确定纯镁（Mg）在大鼠下颌骨的成骨作用及其机制。方法拔除大鼠下颌切牙后，将纯镁植入牙槽内。钛（Ti）用作对照。通过血清镁水平和肝脏和肾脏的组织学分析确定全身效应。局部Mg浓度通过显微镜-能量色散谱（SEM-EDS）测量。通过显微计算机断层扫描（micro-CT）和组织学分析牙槽骨。通过增殖、茜素红染色和定量RT-PCR分析评价0.8至20 mM氯化镁（MgCl 2）对骨膜衍生细胞（PDCs）的成骨作用。结果Mg组和Ti组的全身反应相似。Mg组局部Mg浓度明显高于对照组（P < 0.05）。Micro-CT显示Mg组术后2周和6周的牙槽骨体积较大，松质骨密度较高，H&E染色显示外侧牙槽骨表面有大量新骨膜下骨形成。在PDC培养中，5、10和20 mM MgCl（2）组的增殖率、侏儒相关转录因子2（Runx 2）、骨唾液蛋白（Bsp）和骨钙素（Ocn）的成骨基因表达以及钙结节形成显著升高。结论纯镁可诱导大鼠下颌牙槽骨快速成骨（尤其是骨膜下）。PDCs的成骨能力在体外通过较高的Mg离子浓度增强。
Background Bone augmentation or grafting is often required for placement of dental implants or surgical reconstruction of bony defects. Bioabsorbable magnesium implant was shown to promote osteogenesis in long bones. The objectives of this study were to determine osteogenic effects of pure magnesium (Mg) in rat mandible and underlying mechanisms. Methods Pure Mg was implanted in sockets after rat mandibular incisors were extracted. Titanium (Ti) was used as control. Systemic effects were determined by serum Mg level and histologic analyses of liver and kidney. Local Mg concentration was measured by microscopy-energy-dispersive spectroscopy (SEM-EDS). Alveolar bone was analyzed by micro-computed tomography (micro-CT) and histology. Osteogenic effects of 0.8 to 20 mM magnesium chloride (MgCl2) on periosteum-derived cells (PDCs) were evaluated by proliferation, alizarin red staining and quantitative RT-PCR assays. Results Systemic effects were similar in Mg and Ti groups. Higher local Mg concentration was detected in Mg group (P < 0.05). Micro-CT showed higher alveolar bone volume (2- and 6-weeks post-operation) and denser cancellous bone (2 weeks post-operation) in Mg group, with significant amount of new subperiosteal bone formation on lateral alveolar bone surfaces by H&E staining. In PDC culture, proliferation rates, osteogenic gene expression for runt related transcription factor 2 (Runx2), bone sialoprotein (Bsp) and osteocalcin (Ocn), as well as calcium nodule formation rose significantly in 5, 10, and 20 mM MgCl(2)groups. Conclusions Rapid osteogenesis (especially subperiosteal) is induced by pure Mg in rat mandibular alveolar bone. Osteogenic capacity of PDCs is enhanced by higher Mg ion concentrations in vitro.