A technique for studying the innervation of organs
A technique for studying the innervation of organs
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研究器官神经支配的技术
DOI：
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发表时间：
1937
期刊：
影响因子：
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通讯作者：
L. R. Wharton
中科院分区：
文献类型：
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作者：
L. R. Wharton
我们发表这项技术是因为它在过去的6年里对我们非常有帮助。我们用它解剖了柳麦猴输尿管、肾脏和膀胱的神经供应，以及雌猴猕猴的生殖器官。它特别适用于细神经丛和神经节的研究，血管的神经支配和蒂，以及神经与器官的关系的解剖。这种方法的基本原理是，在解剖组织之前，将组织固定并永久染色。此外，在固定和染色后，组织保持柔软和柔韧，因此可以很容易地解剖。通过这种技术，许多其他组织也会被染色，比如肌肉、血管和结缔组织，但由于神经的染色最深，因此对比鲜明而清晰。NTe在6年前发展了这项技术，当时我们正在寻找一种令人满意的方法来解剖人类输尿管的神经。我们以通常的方式开始对输尿管神经支配的调查，对尸体进行大体解剖，无论是新鲜的还是用福尔马林固定的。然而，6个月后，我们意识到这样的解剖不能满足我们的需求。首先，它们没有显示出精细或精致的结构。即使使用光学透镜，x7e也不能像我们希望的那样准确地分析神经丛、神经节和神经通路。然后，再一次，不可能识别出更精细的结构，并区分神经
TITe are publishing this technique because it has been extremely helpful to us in the past 6 years. We have used it in dissecting the nerve supply of the ureter, kidney and bladder of the liumaii being, and the reproductive organs of the female monkey, Ilacacus rhesus. It is particularly adapted to the study of fine plexuses and ganglia, the innervation of blood vessels aiid pedicles and the dissectioii of nerves in their relation to organs. The priuciple which underlies this method is that the tissue is fixed and stained permanently before it is dissected. After it has been fixed and stained, moreover, the tissue remains soft and pliable, so that it can be dissected easily. By this technique, many other tissues also are stained, such as muscle, blood vessels and connective tissue, but since nerve takes the stain most deeply, the contrast is striking and clear. NTe evolved this technique 6 years ago while we were searching for a satisfactory method of dissecting the nerves of the human ureter. We started our investigation of the ureteral innervation in the usual way, making gross dissections of cadavers, either fresh or fixed by formalin. After 6 months, however, we realized that such dissections would not answer our needs. Iii the first place, they did not reveal fine a id delicate structures. Even when using optical lenses, x7e could not snalyse plexuses, ganglia and nerve pathways as accurately as we wished to. Then, again, it was impossible to identify the iiature of finer structures, and distinguish nerves