Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid
Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid
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DOI：
10.1007/s12038-013-9308-9
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发表时间：
2013-06-01
影响因子：
2.9
通讯作者：
Chattopadhyay, Sharmila
中科院分区：
文献类型：
--
作者：
Datta, Riddhi;Sinha, Ragini;Chattopadhyay, Sharmila
水杨酸（SA）参与了许多植物与病原菌相互作用的结果。在转录水平上已经观察到这种植物激素的全球变化，但很少有人知道它是如何诱导蛋白质丰度的变化。为此，我们研究了1 mM SA对拟南芥叶片可溶性蛋白质的影响，通过蛋白质组学分析。对时间景观进行了转录水平的初步研究，结果表明大多数SA响应基因的诱导发生在处理后3至6小时（HPT）内，并在24 HPT内达到表达峰值。采用双向凝胶电泳（2-DE）结合MALDI-TOF MS/MS技术对差异表达蛋白进行鉴定，共鉴定出63个蛋白点。SA处理的叶片与对照叶片的比较蛋白质组学分析表明，许多防御相关蛋白如致病相关蛋白10 a（PR 10 a）、抗病样蛋白、推定的晚疫病抗性蛋白、WRKY 4、MYB 4等的变化沿着能量产生速率的总体增加，而其他一般代谢速率略有降低，这大概意味着从“正常模式”到“防御模式”的转变。
Salicylic acid (SA) has been implicated in determining the outcome of interactions between many plants and their pathogens. Global changes in response to this phytohormone have been observed at the transcript level, but little is known of how it induces changes in protein abundance. To this end we have investigated the effect of 1 mM SA on soluble proteins of Arabidopsis thaliana leaves by proteomic analysis. An initial study at transcript level has been performed on temporal landscape, which revealed that induction of most of the SA-responsive genes occurs within 3 to 6 h post treatment (HPT) and the expression peaked within 24 HPT. Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF MS/MS analysis has been used to identify differentially expressed proteins and 63 spots have been identified successfully. This comparative proteomic profiling of SA treated leaves versus control leaves demonstrated the changes of many defence related proteins like pathogenesis related protein 10a (PR10a), disease-resistance-like protein, putative late blight-resistance protein, WRKY4, MYB4, etc. along with gross increase in the rate of energy production, while other general metabolism rate is slightly toned down, presumably signifying a transition from 'normal mode' to 'defence mode'.