Descemet membrane endothelial keratoplasty (DMEK)
Descemet membrane endothelial keratoplasty (DMEK)
复制标题
碱诱导的角膜新生血管形成与 CXCR2 介导的中性粒细胞浸润无关
DOI：
10.1097/01.ico.0000248385.16896.34
复制
发表时间：
2006-09-01
期刊：
影响因子：
2.8
通讯作者：
van der Wees, Jacqueline
中科院分区：
文献类型：
--
作者：
Melles, Gerrit R. J.;Ong, T. San;van der Wees, Jacqueline
目的：目的：在人尸体眼模型和Fuchs角膜内皮营养不良患者眼模型上，采用器官培养后弹力层（DM）行角膜内皮移植术（DMEK）。方法：在10只人尸体眼和1例患者眼上，制作3.5mm透明角膜隧道切口。前房充满空气，DM从后基质剥离。从器官培养的供体角膜巩膜缘，收获9.0 mm直径的“DM卷”。每个捐助者DM卷插入到一个受体前房，定位到后基质，并保持在位置完全充满前房空气30 minutes.Results：在所有受体的眼睛，捐助者DM保持其位置后30分钟的空气填充的前房，然后由空气液体交换。术后1周，患者最佳矫正视力为1.0（20/20），角膜内皮细胞密度平均为2350 cells/mm 2。结论：DMEK通过透明角膜隧道切口移植器官培养的DM，可快速恢复角膜内皮功能。DMEK可能是角膜外科医生高度可及的程序，因为供体DM片可以从保存的角膜巩膜缘制备。
Purpose: To describe Descemet membrane endothelial keratoplasty (DMEK) with organ Cultured Descemet membrane (DM) in a human cadaver eye model and a patient with Fuchs endothelial dystrophy.Methods: In 10 human cadaver eyes and 1 patient eye, a 3.5-mm clear corneal tunnel incision was made. The anterior chamber was filled with air, and the DM was stripped off from the posterior stroma. From organ-cultured donor corneo-scleral rims, 9.0-mm-diameter "DM rolls" were harvested. Each donor DM roll was inserted into a recipient anterior chamber, positioned onto the posterior stroma, and kept in position by completely filling the anterior chamber with air for 30 minutes.Results: In all recipient eyes, the donor DM maintained its position after a 30-minute air-fill of the anterior chamber followed by an air liquid exchange. In the patient's eye, 1 week after transplantation, best-corrected visual acuity was 1.0 (20/20) with the patient's preoperative refraction, and the endothelial cell density averaged 2350 cells/mm(2).Conclusion: DMEK may provide quick visual rehabilitation in the treatment of corneal endothelial disorders by transplantation of an organ-cultured DM transplanted through a clear corneal tunnel incision. DMEK may be a highly accessible procedure to corneal surgeons, because donor DM sheets can be prepared from preserved corneo-scleral rims.