Preparation of silicon nitride biochips for reflectometric interference spectroscopic (RIfS) analysis of biological toxins and E. coli O157:H7 strain
Preparation of silicon nitride biochips for reflectometric interference spectroscopic (RIfS) analysis of biological toxins and E. coli O157:H7 strain
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DOI：
10.1016/j.snb.2017.02.136
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发表时间：
2017-07
影响因子：
8.4
通讯作者：
Takehiro Nagatsuka;H. Uzawa;Daiki Tanaka;Y. Oba;Y. Nishida;T. Iwasa;K. Tayama;Toshio Yoshida;T. Ezaki;Y. Seto
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文献类型：
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作者：
Takehiro Nagatsuka;H. Uzawa;Daiki Tanaka;Y. Oba;Y. Nishida;T. Iwasa;K. Tayama;Toshio Yoshida;T. Ezaki;Y. Seto
用金（Au）纳米颗粒，然后用乳糖基衍生物或单克隆抗体对市售氮化硅（SiN）衬底的表面进行改性。利用反射干涉光谱法（RIfS）对蓖麻凝集素（RCA 120）和毒素（RCA 60）进行了分析。使用Au-乳糖/SiN芯片的RIfS分析使我们能够检测浓度小于5-10 ng/mL的植物凝集素以及浓度约为1 μg/mL的测试样品中仔细密封的毒素（蓖麻毒素）。应用金抗体/SiN芯片的RIfS分析方法，可在可能存在干扰物质的情况下，简便、直接地筛选肠出血性大肠杆菌（EHEC）O 157：H7菌株。整个检测在2.5 h培养时间内完成，无需对病原菌进行任何纯化过程。
The surface of a commercially available silicon nitride (SiN) substrate was modified with gold (Au) nanoparticle and then with either lactosyl derivative or monoclonal antibody. Each of the derived SiN chips carrying Au-lactose or Au-antibody was evaluated in the reflectometric interference spectroscopic (RIfS) analyses ofRicinus communislectin (RCA120) and toxin (RCA60). The RIfS analysis using the Au-lactose/SiN chip enabled us to detect the plant lectin at concentrations less than 5–10 ng/mL as well as the toxin (ricin) sealed carefully in a test sample at concentration about 1 μg/mL. The RIfS analysis using the Au-antibody/SiN chip was useful for the facile and direct screening of enterohemorrhagicEscherichia coli(EHEC) O157:H7 strain in the presence of possible interfering materials. The overall detection was completed within 2.5 h-cultivation time without any purification process for the pathogenic bacterium.