The Preconditioning of Busulfan Promotes Efficiency of Human CD133+Cells Engraftment in NOD Shi-SCID IL2Rγcnull (NOG) Mice via Intra-Bone Marrow Injection
The Preconditioning of Busulfan Promotes Efficiency of Human CD133+Cells Engraftment in NOD Shi-SCID IL2Rγcnull (NOG) Mice via Intra-Bone Marrow Injection
复制标题
白消安预处理通过骨髓内注射促进人 CD133 细胞在 NOD Shi-SCID IL2R gamma cnull (NOG) 小鼠中的植入效率
DOI：
10.1177/0963689719842162
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发表时间：
2019-07-01
影响因子：
3.3
通讯作者：
Zhu, Xiaofei
中科院分区：
文献类型：
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作者：
Guo, Xiaofang;Yin, Xiaoxiao;Zhu, Xiaofei
将人CD133(+)干细胞注射到NOG(NOD SHI-SCID IL2R Gamma c(Null))小鼠的骨髓腔内，观察其对人CD133(+)细胞的植入效率。对移植CD133(+)的NOG小鼠外周血进行流式细胞仪分析。结果表明，移植后4周可检测到人CD19(+)B淋巴细胞，移植后16周可检测到人CD4(+)、CD8(+)T淋巴细胞亚群、CD19(-)CD14(-)、HLA-DR+DC和CD19(-)CD14(+)单核细胞。未用药组小鼠存活率(100%)略高于用药组(83%)(P>；0.05)。而白术前处理组CD133(+)干细胞的分化效率明显高于未处理组(P<；0.05)。提示CD133(+)细胞可作为人源化小鼠模型的良好来源，白术前的预适应更有利于骨髓内注射促进人CD133(+)细胞在NOG小鼠体内的分化。
Human CD133(+) stem cells were injected into the bone marrow cavity of NOG (NOD Shi-SCID IL2R gamma c(null)) mice with or without preconditioning of busulfan in order to assess the efficiency of human CD133(+) cells engraftment. Peripheral blood from CD133(+)-engrafted NOG mice was analyzed by flow cytometry. The results showed that human CD19(+) B lymphocytes could be detected at 4 weeks post-transplantation, and human CD4(+), CD8(+) subsets of T lymphocytes, CD19(-) CD14(-) HLA-DR+ DCs and CD19(-) CD14(+) monocytes could be detected at 16 weeks post-transplantation. The survival rate of mice in busulfan-untreated group (100%) was slightly higher than that in the busulfan-pretreated group (83%) (P > 0.05). However, the differentiation efficiency of CD133(+) stem cells in busulfan-pretreated group was significantly higher than that in the untreated group (P < 0.05). This data imply that CD133(+) cells could be a good resource for a humanized mouse model, and the preconditioning of busulfan could be more conducive to accelerating the differentiation of human CD133(+) cells in NOG mice by intra-bone marrow injection.