Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell.
Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell.
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Amphibalanus（= Balanus）安菲特里特壳的化学成分和蛋白质组学研究
DOI：
10.1371/journal.pone.0133866
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Qian PY
中科院分区：
文献类型：
--
作者：
Zhang G;He LS;Wong YH;Xu Y;Zhang Y;Qian PY
藤壶是典型的生物污损生物，具有坚硬的外壳，造成严重的生物污损问题。在这项研究中，我们分析了藤壶Amphibalanus（=藤壶）amphitrite壳蛋白质组成的凝胶蛋白质组学。结果表明，A. Amphitrite外壳。其中40个蛋白质在KOG数据库中被分为11个功能组，其余12个蛋白质未知。除了已知的藤壶壳蛋白（SIPC、碳酸酐酶和酸性基质蛋白）外，我们还鉴定了绒膜过氧化物酶、C型凝集素样结构域、丝氨酸蛋白酶和蛋白酶抑制剂蛋白。Amphitrite外壳。这些蛋白质的序列进行了表征，并讨论了其潜在的功能。组织学和DAPI染色显示壳中有活细胞，这些细胞可能分泌本研究中鉴定的壳蛋白。
As typical biofoulers, barnacles possess hard shells and cause serious biofouling problems. In this study, we analyzed the protein component of the barnacle Amphibalanus (= Balanus) amphitrite shell using gel-based proteomics. The results revealed 52 proteins in the A. Amphitrite shell. Among them, 40 proteins were categorized into 11 functional groups based on KOG database, and the remaining 12 proteins were unknown. Besides the known proteins in barnacle shell (SIPC, carbonic anhydrase and acidic acid matrix protein), we also identified chorion peroxidase, C-type lectin-like domains, serine proteases and proteinase inhibitor proteins in the A. Amphitrite shell. The sequences of these proteins were characterized and their potential functions were discussed. Histology and DAPI staining revealed living cells in the shell, which might secrete the shell proteins identified in this study.