Silver nanoparticle characterization using single particle ICP-MS (SP-ICP-MS) and asymmetrical flow field flow fractionation ICP-MS (AF4-ICP-MS)
Silver nanoparticle characterization using single particle ICP-MS (SP-ICP-MS) and asymmetrical flow field flow fractionation ICP-MS (AF4-ICP-MS)
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DOI：
10.1039/c2ja30021d
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发表时间：
2012-01-01
影响因子：
3.4
通讯作者：
Ranville, James F.
中科院分区：
文献类型：
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作者：
Mitrano, Denise M.;Barber, Angela;Ranville, James F.
在环境基质中检测、量化和表征工程纳米颗粒(ENPs)的方法被强调为了解与纳米材料相关的潜在环境风险方面的最优先研究需要之一。更具体地说，需要技术来确定ENPs在各种复杂基质中的大小和浓度。此外，应以与环境和毒物相关的浓度收集数据。与显微镜、光散射和过滤等传统表征方法相比，单颗粒电感耦合等离子体质谱(SP-ICP-MS)和不对称流场流动分离(AF4)电感耦合等离子体质谱(AF4)在检测ENPs和评估复杂基质中的上述许多参数方面都具有显著的优势。在这项研究中，我们比较了两种新兴技术检测特征良好的单分散银ENPs的能力，并考察了它们在环境研究中的总体适用性，特别是在它们的以下方面：(A)大小和浓度检测限、(B)分辨率和(C)多种元素分析。我们发现，在浓度检测下限(质量和粒子数量)方面，SP-ICPMS比AF4-ICPMS灵敏得多(分别为ng L-1和mUg L-1)，并提供了区分总金属中溶解和纳米颗粒的独特能力。通过各种可能的优化参数，AF4-ICP-MS可以检测到更小的NP尺寸(SP-ICP-MS为2 nm比20 nm)，为更高的尺寸分辨率提供了可能性。
Methods to detect, quantify, and characterize engineered nanoparticles (ENPs) in environmental matrices are highlighted as one of the areas of highest priority research needs with respect to understanding the potential environmental risks associated with nanomaterials. More specifically, techniques are needed to determine the size and concentration of ENPs in a variety of complex matrices. Furthermore, data should be collected at environmentally and toxicologically relevant concentrations. Both single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) and asymmetrical flow field flow fractionation (AF4) ICP-MS offer substantial advantages for detecting ENPs and assessing many of the above parameters in complex matrices over traditional characterization methods such as microscopy, light scattering, and filtration. In this study, we compared the ability of two emerging techniques to detect well characterized, monodisperse silver ENPs and examined their overall applicability to environmental studies specifically with respect to their: (A) size and concentration detection limits, (B) resolution and (C) multi-form elemental analysis. We find that in terms of concentration detection limit (both, on a mass basis and particle number basis) SP-ICP-MS was considerably more sensitive than AF4-ICP-MS (ng L-1 vs. mu g L-1, respectively), and offers the unique ability to differentiate dissolved and nanoparticulate fractions of total metal. With a variety of optimization parameters possible, AF4-ICP-MS can detect a much smaller NP size (2 nm vs. 20 nm for SP-ICP-MS), provides the possibility for greater size resolution.