Phylogenetic framework of the systematically confused Anteholosticha–Holosticha complex (Ciliophora, Hypotrichia) based on multigene analysis
Phylogenetic framework of the systematically confused Anteholosticha–Holosticha complex (Ciliophora, Hypotrichia) based on multigene analysis
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基于多基因分析的系统性混淆的 Anteholosticha-Holosticha 复合体（Ciliophora、Hypotrichia）的系统发育框架
DOI：
10.1016/j.ympev.2015.05.021
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发表时间：
2015
影响因子：
4.1
通讯作者：
Jie Huang
中科院分区：
文献类型：
--
作者：
Xiaolu Zhao;Shan Gao;Yangbo Fan;Michaela Strueder-Kypke;Jie Huang
Anteholosticha-HolostichacComplex在尾类中是一个非常不同的类群，特别是因为属的特征缺乏合适的同形异形。以往的研究表明，该群内物种的形态分类经常与SSU-rDNA数据相冲突，即该复合体没有恢复为一个单系群和Anteholostichaspp。广泛分布在SSU-rDNA树中的尿柄内酯组合中。在这项研究中，我们提供了38个新的SSU-rDNA、ITS1-5.8S-ITS2和LSU-rDNA基因序列(包括Anteholosticha模式种)，以推测Anteholosticha-Holosticha复合体中所有可用的分类群的分子系统发育。结果表明：(1)Anteholostichaa在所有树木中都是单系进化的，表明它是一个定义明确的属；(2)Anteholostichaa是多系统发育的，在所有基于单基因和连锁的系统发育中与Holostichaa明显分开；(3)从Anteholosticha分裂出来的一个新属Arcus Series的单系性得到了所有分子数据的有力支持；(4)Anteholosticha MultiCirrata、Anteholosticha Manca、Anteholosticha parancanca和Bakuticella subtropicamam可能共享一个最新的共同祖先；(5)多基因分析得到的支持度高于单基因分析。
TheAnteholosticha–Holostichacomplex is an extremely divergent group within the urostylids, especially because the genus characterization lacks suitable synapomorphies. Previous studies have shown that morphological classification of species within this group often conflicts with SSU-rDNA data, that is this complex is not recovered as a monophyletic group andAnteholostichaspp. are widely dispersed throughout the urostylid assemblage in SSU-rDNA trees. In this study, we provided 38 new sequences (including the type species ofAnteholosticha) of SSU-rDNA, ITS1-5.8S-ITS2 and LSU-rDNA genes to infer molecular phylogenies of all available taxa in theAnteholosticha–Holostichacomplex. The results show that: (1)Holostichais monophyletic in all trees, suggesting it is a well-defined genus; (2)Anteholostichais polyphyletic and distinctly separated fromHolostichain all single-gene based and concatenated phylogenies; (3) the monophyly ofArcuseries, a recently established genus split fromAnteholosticha, is strongly supported by all molecular data; (4)Anteholosticha multicirrata,Anteholosticha manca,Anteholosticha paramancaandBakuella subtropicamay share a most recent common ancestor; (5) multi-gene analyses receive higher support values than the single-gene analyses.