Analysis of MicroRNA in Bile Cytologic Samples Is Useful for Detection and Diagnosis of Extrahepatic Cholangiocarcinoma
Analysis of MicroRNA in Bile Cytologic Samples Is Useful for Detection and Diagnosis of Extrahepatic Cholangiocarcinoma
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胆汁细胞学样本中的 MicroRNA 分析有助于肝外胆管癌的检测和诊断
DOI：
10.1093/ajcp/aqac015
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发表时间：
2022
影响因子：
3.5
通讯作者：
Ishida Katsunari
中科院分区：
文献类型：
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作者：
Uchihata Yukari;Arihiro Koji;Kaneko Yoshie;Shimizu Tomomi;Marubashi Yukari;Aoki Chie;Murakami Takuya;Ochi Mayu;Niihara Nanaka;Ohtsuka Kohei;Unehara Rimu;Araki Yusuke;Seki Yoshinaga;Mori Keiichi;Oda Miyo;Ishida Katsunari
ObjectivesThis study aimed to develop reliable biomarkers that improve bile cytology to diagnose cholangiocarcinoma vs benign biliary lesions.MethodsMany studies indicate that microRNAs（miRNAs）are potential candidates for early diagnosis of cancer.我们使用定量逆转录聚合酶链反应分析了细胞学样本中5种肿瘤相关miRNA（miR-31 - 5p、miR-122 - 5p、miR-378d、miR-182 - 5p和miR-92a-3p）的表达。我们收集了52例手术切除的组织标本，84例涂片细胞学标本结果miR-31 - 5p、miR-378d和miR-122 - 5p在癌组织中的表达显著高于正常组织，在正常组织中的表达显著高于癌旁组织，在癌旁组织中的表达显著高于癌旁组织，在癌旁组织中的表达显著高于癌旁组织，在癌旁组织中的表达显著高于癌旁组织，在癌旁组织中的表达显著高于癌旁组织。而miR-182 - 5p表达较低。miR-31 - 5p，miR-378d，miR-182 - 5p，和miR-92a-3p的表达显着高于脱落的细胞样本从胆管癌的情况下涂片比那些从noncancer cases. ConclusionsThese结果表明，在胆汁细胞学标本中的miRNA的分析是一个有前途的辅助工具，用于区分胆管癌良性胆道病变。
ObjectivesThis study aimed to develop reliable biomarkers that improve the ability of bile cytology to diagnose cholangiocarcinoma vs benign biliary lesions.MethodsMany studies indicate that microRNAs (miRNAs) are potential candidates for the early diagnosis of cancer. We analyzed the expression of five tumor-associated miRNAs (miR-31-5p, miR-122-5p, miR-378d, miR-182-5p, and miR-92a-3p) in cytology samples using quantitative reverse transcription polymerase chain reaction. We collected 52 surgically resected tissue samples, 84 cytologic specimens from smears (53 cases of cancer and 31 cases of noncancer), and 40 residual sediments after smearing for routine cytology at Hiroshima University Hospital.ResultsThe expression of miR-31-5p, miR-378d, and miR-122-5p was significantly higher in cancer tissues than those in normal tissues, while miR-182-5p expression was lower. The expression of miR-31-5p, miR-378d, miR-182-5p, and miR-92a-3p was significantly higher in detached cell samples from smears of cholangiocarcinoma cases than in those from noncancer cases.ConclusionsThese results suggest that the analysis of miRNAs in bile cytologic specimens is a promising auxiliary tool for distinguishing cholangiocarcinoma from benign biliary lesions.