The Dynamics of P Granule Liquid Droplets Are Regulated by the Caenorhabditis elegans Germline RNA Helicase GLH-1 via Its ATP Hydrolysis Cycle
The Dynamics of P Granule Liquid Droplets Are Regulated by the Caenorhabditis elegans Germline RNA Helicase GLH-1 via Its ATP Hydrolysis Cycle
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DOI：
10.1534/genetics.120.303052
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发表时间：
2020-06-01
期刊：
影响因子：
3.3
通讯作者：
Lee, Heng-Chi
中科院分区：
文献类型：
--
作者：
Chen, Wenjun;Hu, Yabing;Lee, Heng-Chi
P颗粒是在秀丽隐杆线虫生殖细胞命运维持中起重要作用的相分离液滴。P颗粒的定位和形成都是高度动态的，但调节这些过程的机制仍然知之甚少。在这里，我们证明了VASA样种系RNA解旋酶夫妇的ATP酶水解周期的不同步骤，以控制P颗粒的形成和拆卸的证据。此外，我们还发现苯丙氨酸-甘氨酸-甘氨酸重复序列促进了它在核周的定位。蛋白质组学分析的复杂的突变，干扰P颗粒解体揭示了短暂的相互作用与几个Argonaute和RNA结合蛋白。最后，我们发现，缺陷招募的P颗粒成分的核周灶在成年生殖细胞与生育缺陷中观察到的各种突变体。总之，我们的研究结果突出了RNA解旋酶在控制液滴在空间和时间上的形成方面的多功能作用。
P granules are phase-separated liquid droplets that play important roles in the maintenance of germ cell fate in Caenorhabditis elegans. Both the localization and formation of P granules are highly dynamic, but mechanisms that regulate such processes remain poorly understood. Here, we show evidence that the VASA-like germline RNA helicase couples distinct steps of its ATPase hydrolysis cycle to control the formation and disassembly of P granules. In addition, we found that the phenylalanine-glycine-glycine repeats in promote its localization at the perinucleus. Proteomic analyses of the complex with a mutation that interferes with P granule disassembly revealed transient interactions of with several Argonautes and RNA-binding proteins. Finally, we found that defects in recruiting the P granule component to perinuclear foci in the adult germline correlate with the fertility defects observed in various mutants. Together, our results highlight the versatile roles of an RNA helicase in controlling the formation of liquid droplets in space and time.