CONFOCAL MICROSCOPY WITH AN INCREASED DETECTION APERTURE - TYPE-B 4PI CONFOCAL MICROSCOPY
CONFOCAL MICROSCOPY WITH AN INCREASED DETECTION APERTURE - TYPE-B 4PI CONFOCAL MICROSCOPY
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DOI：
10.1364/ol.19.000222
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发表时间：
1994-02-01
期刊：
影响因子：
3.6
通讯作者：
CREMER, C
中科院分区：
文献类型：
--
作者：
HELL, SW;STELZER, EHK;CREMER, C
我们给出了增加探测孔径的共焦显微镜的点扩散函数。孔径的增加是通过用两个相对的物镜，即B4PI共焦显微镜，相干地收集来自样品的光来实现的。我们在实验上证明了它用于检测散射光或荧光发射光的可行性。给出了4PI共焦点扩展函数，并与同类共焦显微镜的点扩展函数进行了比较。
We show the point-spread function of a confocal microscope with an increased detection aperture. The increase in aperture is accomplished by coherent collection of the light from the specimen with two opposing objective lenses, i.e., type-B 4Pi confocal microscopy. We demonstrate experimentally its feasibility for detecting scattered or fluorescently emitted light. The 4Pi confocal point-spread functions are shown for constructive and destructive interference of the collected wave fronts and are compared with the point-spread functions of comparable confocal microscopes.