Cross Talk between ARF1 and RhoA Coordinates the Formation of Cytoskeletal Scaffolds during Chlamydia Infection.
Cross Talk between ARF1 and RhoA Coordinates the Formation of Cytoskeletal Scaffolds during Chlamydia Infection.
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DOI：
10.1128/mbio.02397-21
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发表时间：
2021-12-21
期刊：
影响因子：
6.4
通讯作者：
Paumet F
中科院分区：
文献类型：
--
作者：
Haines A;Wesolowski J;Ryan NM;Monteiro-Brás T;Paumet F
沙眼衣原体是一种专性细胞内细菌，已发展出复杂的机制，以生存在其感染区室，包括。值得注意的是，衣原体编织了一个广泛的微管（MT）和肌动蛋白丝网络，使其能够与宿主细胞器相互作用，并提高其稳定性。尽管这种性传播病原体造成了全球健康和经济负担，但人们对肌动蛋白和MT支架如何整合到日益复杂的毒力系统中知之甚少。以前，我们建立了衣原体效应InaC与ARF1相互作用，以稳定MT。我们现在证明InaC调节RhoA以控制肌动蛋白支架。InaC依赖于ARF1和RhoA之间的串扰来协调MT和肌动蛋白，其中RhoA的存在下调稳定的MT支架，而ARF1激活抑制肌动蛋白支架。了解衣原体如何劫持复杂的网络将有助于阐明这种临床上重要的病原体如何寄生于其宿主，并揭示新的细胞信号通路。
Chlamydia trachomatis is an obligate intracellular bacterium that has developed sophisticated mechanisms to survive inside its infectious compartment, the inclusion. Notably, Chlamydia weaves an extensive network of microtubules (MTs) and actin filaments to enable interactions with host organelles and enhance its stability. Despite the global health and economic burden caused by this sexually transmitted pathogen, little is known about how actin and MT scaffolds are integrated into an increasingly complex virulence system. Previously, we established that the chlamydial effector InaC interacts with ARF1 to stabilize MTs. We now demonstrate that InaC regulates RhoA to control actin scaffolds. InaC relies on cross talk between ARF1 and RhoA to coordinate MTs and actin, where the presence of RhoA downregulates stable MT scaffolds and ARF1 activation inhibits actin scaffolds. Understanding how Chlamydia hijacks complex networks will help elucidate how this clinically significant pathogen parasitizes its host and reveal novel cellular signaling pathways.