Development of a loop-mediated isothermal amplification assay for rapid Helicobacter pylori detection.
Development of a loop-mediated isothermal amplification assay for rapid Helicobacter pylori detection.
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DOI：
10.1016/j.mimet.2019.105653
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发表时间：
2019-08-01
影响因子：
2.2
通讯作者：
Yano, Hisakazu
中科院分区：
文献类型：
--
作者：
Horiuchi, Saori;Nakano, Ryuichi;Yano, Hisakazu
cagA阳性幽门螺杆菌感染与胃癌相关分子生物学技术对于精确的H。pylori诊断我们建立了环介导等温扩增（LAMP）方法检测H。pylori cagA基因的检测及其在临床诊断中的应用。设计了一对LAMP引物，用于识别6 H. pylori菌株。用H. pylori ATCC 43504和粪便标本，用H. pylori ATCC 49396和CIP 104086。H. pylori标本的检测灵敏度为10- 1cfu/管（反应时间37 min），比聚合酶链反应灵敏10倍。LAMP对粪便标本也具有高度的敏感性和快速性。检测ATCC 49396和CIP 104086的cagA基因。提示LAMP可用于H.幽门螺杆菌感染，以降低胃癌发病率。
Infection with cagA-positive Helicobacter pylori is associated with gastric cancer. Molecular techniques are vital for accurate H. pylori diagnosis. We developed a loop-mediated isothermal amplification (LAMP) for detecting the H. pylori cagA gene and evaluated its use for clinical diagnosis. A LAMP primer set was designed to recognize the homologous regions of cagA gene sequences of 6 H. pylori strains. LAMP sensitivity was evaluated with serial dilutions of H. pylori ATCC 43504 and fecal specimens; specificity was evaluated with H. pylori ATCC 49396 and CIP 104086. The LAMP sensitivity for H. pylori specimens was 10-1 cfu/tube (reaction time, 37 min), which was 10-fold more sensitive than polymerase chain reaction. LAMP was also highly sensitive and rapid for fecal specimens. It detected cagA gene from ATCC 49396 and CIP 104086. The findings suggest LAMP can be used for diagnosing and screening of H. pylori infections to decrease gastric cancer incidence.