Gonadal expression profiles of sex-specific genes during early sexual differentiation in Japanese eel Anguilla japonica
Gonadal expression profiles of sex-specific genes during early sexual differentiation in Japanese eel Anguilla japonica
复制标题
DOI：
10.1007/s12562-020-01491-5
复制
发表时间：
2021-01-12
影响因子：
1.9
通讯作者：
Kitano, Takeshi
中科院分区：
文献类型：
--
作者：
Inaba, Hiroyuki;Hara, Seiji;Kitano, Takeshi
日本鳗鲡（Anguilla japonica）是日本水产养殖业中的重要且高价值物种。鳗鱼的性别决定和性别分化受环境因素影响显着；然而，鳗鱼性别分化的分子机制尚不清楚。在这项研究中，为了研究日本鳗鱼性别分化期间和性别分化后性别特异性基因的性腺表达谱，我们通过在性别分化期间分别采用对照饮食或雌二醇-17β处理饮食饲养，将鳗鱼诱导为主要表型的雄性或雌性。实时定量PCR显示，叉头盒L2A（foxl2a）和细胞色素P450芳香酶（cyp19a1）在卵巢中的表达水平高于睾丸，而抗苗勒管激素（amh）和性腺胞体衍生因子（gsdf）的表达水平在睾丸中显着高于卵巢。此外，foxl2a和cyp19a1在性别分化过程早期显示出雌性特异性表达，而在稍微生长更多后，amh和gsdf在性别分化过程中显示出雄性特异性表达。总之，这些结果表明这些基因在该物种的性分化和发育中具有重要作用。
The Japanese eel Anguilla japonica is a prominent and highly valued species in the aquaculture industry in Japan. Sex determination and sex differentiation in eels are significantly affected by environmental factors; however, the molecular mechanisms involved in sex differentiation in eels are largely unknown. In this study, to investigate the gonadal expression profiles of sex-specific genes during and after sexual differentiation in Japanese eel, we induced elvers into predominantly phenotypic males or females by rearing on a control diet or estradiol-17 beta-treated diet, respectively, during the sex differentiation period. Quantitative real-time PCR showed that forkhead box L2A (foxl2a) and cytochrome P450 aromatase (cyp19a1) were more highly expressed in ovaries than in testes, whereas the expression levels of anti-Mullerian hormone (amh) and gonadal soma-derived factor (gsdf) were significantly higher in testes than in ovaries. Furthermore, foxl2a and cyp19a1 displayed female-specific expression early in the sex differentiation process, while after slightly more growth amh and gsdf displayed male-specific expression during sex differentiation. Together, these results suggest that these genes have important roles in sexual differentiation and development in this species.