Chlamydia trachomatis detected in human placenta
Chlamydia trachomatis detected in human placenta
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DOI：
10.1136/jcp.50.10.852
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发表时间：
1997-10-01
影响因子：
3.4
通讯作者：
Koskiniemi, ML
中科院分区：
文献类型：
--
作者：
Gencay, M;Puolakkainen, M;Koskiniemi, ML
目的 - 为了评估沙眼衣原体感染与死产之间的关系，研究胎盘组织中是否存在沙眼衣原体。方法 - 研究了 21 岁母亲在妊娠第 36 周出生的死产胎儿的石蜡包埋胎盘组织，该母亲在怀孕期间血清沙眼衣原体免疫型抗体滴度较高，且三年前沙眼衣原体培养呈阳性。原位杂交、聚合酶链式反应和免疫组织化学检测沙眼衣原体的存在。结果-通过原位杂交和多个切片的碱性磷酸酶抗碱性磷酸酶染色在胎盘标本中检测到沙眼衣原体，而对照组织均呈阴性，表明胎盘中存在沙眼衣原体核酸和抗原。结论-这是首次报道沙眼衣原体在胎盘中被证实的病例。人类胎盘。
Aims-To evaluate the relation between Chlamydia trachomatis infection and stillbirth, placental tissue was studied for the presence of C trachomatis.Methods-Paraffin wax embedded placental tissue of a stillbirth fetus, born at the 36th week of gestation to a 21 year old mother with high serum antibody titres to C trachomatis immunotypes during pregnancy and who was culture positive to C trachomatis three years previously, was studied by in situ hybridisation, polymerase chain reaction, and immunohistochemistry for the presence of C trachomatis.Results-C trachomatis was detected in placental specimens by in situ hybridisation and alkaline phosphatase antialkaline phosphatase staining in several sections, whereas control tissues were uniformly negative, indicating the presence of C trachomatis nucleic acid and antigen in the placenta.Conclusion-This is the first reported case in which C trachomatis has been demonstrated in the human placenta.