Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography.
Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography.
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DOI：
10.1364/ol.40.004911
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发表时间：
2015-11-01
期刊：
影响因子：
3.6
通讯作者：
Srinivasan VJ
中科院分区：
文献类型：
--
作者：
Chong SP;Merkle CW;Cooke DF;Zhang T;Radhakrishnan H;Krubitzer L;Srinivasan VJ
建立了一种光谱/傅立叶域光学相干断层成像（OCT）活体显微镜，采用1.7 μm超连续谱光源，对活体小鼠脑皮质下结构进行了无创性研究。通过定量比较可见光和近红外波长范围内皮质组织的OCT信号衰减特性，证明了1.7 μm用于深部组织脑成像的优势。海马组织结构和白色物质微血管成像显示在体内通过薄颅骨，玻璃盖玻片加强颅窗小鼠。这种新型平台的应用包括监测阿尔茨海默病和皮质下痴呆（包括血管性痴呆）啮齿动物模型中的疾病进展和病理生理学。
A spectral/Fourier domain optical coherence tomography (OCT) intravital microscope using a supercontinuum light source at 1.7 μm was developed to study subcortical structures noninvasively in the living mouse brain. The benefits of 1.7 μm for deep tissue brain imaging are demonstrated by quantitatively comparing OCT signal attenuation characteristics of cortical tissue across visible and near-infrared wavelengths. Imaging of hippocampal tissue architecture and white matter microvasculature are demonstrated in vivo through thinned-skull, glass coverslip-reinforced cranial windows in mice. Applications of this novel platform include monitoring disease progression and pathophysiology in rodent models of Alzheimer’s disease and subcortical dementias, including vascular dementia.