Crossed-beam liquid chromatoraph—mass spectrometer combination
Crossed-beam liquid chromatoraph—mass spectrometer combination
复制标题
交叉束液相色谱-质谱联用仪
DOI：
10.1016/s0021-9673(00)89972-0
复制
发表时间：
1978
影响因子：
4.1
通讯作者：
M. Vestal
中科院分区：
文献类型：
--
作者：
C. R. Blakley;M. J. McAdams;M. Vestal
介绍了一种液相色谱-质谱联用的新方法，该方法利用激光汽化液相色谱（LC）出水和分子束技术来传输和电离样品。这种方法的一个关键特点是从LC洗脱的样品被蒸发、电离和质量分析，与固体表面的接触最小。结果表明，交叉束系统的性能相对挥发性芳烃和组分的核酸-碱基，核苷和核苷酸提供了一系列有趣的生物重要化合物的挥发性降低和相应的难度增加质谱分析。给出了在反相和离子交换条件下对LC出水样品进行激光汽化的质谱图。
A new approach to combined liquid chromatograph—mass spectrometry is described which uses laser vaporization of the liquid chromatographic (LC) effluent and molecular beam techniques to transport and ionize the sample. A key feature of this approach is that samples eluting from the LC are vaporized, ionized, and mass analyzed with minimal contact with solid surfaces. Results are presented on the performance of the crossed-beam system for relatively volatile aromatic hydrocarbons and for components of nuclei acids—bases, nucleosides, and nucleotides— which provide an interesting series of biologically important compounds of decreasing volatility and corresponding increasing difficulty for mass spectrometry. Mass spectra are presented which were obtained by laser vaporization of samples from the LC effluent under both reversed-phase and ion-exchange conditions.