ASSOCIATION OF VIRUS-LIKE PARTICLES WITH FOREGUT OF APHID CAVARIELLA-AEGOPODII TRANSMITTING SEMI-PERSISTENT VIRUSES ANTHRISCUS YELLOWS AND PARSNIP YELLOW FLECK
ASSOCIATION OF VIRUS-LIKE PARTICLES WITH FOREGUT OF APHID CAVARIELLA-AEGOPODII TRANSMITTING SEMI-PERSISTENT VIRUSES ANTHRISCUS YELLOWS AND PARSNIP YELLOW FLECK
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DOI：
10.1099/0022-1317-31-1-47
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发表时间：
1976-01-01
影响因子：
3.8
通讯作者：
ELNAGAR, S
中科院分区：
文献类型：
--
作者：
MURANT, AF;ROBERTS, IM;ELNAGAR, S
AYV和PYFV是一种半持续性传播的病毒，在蜕皮时传播消失。它只在携带AYV时才传输PYFV。在感病植物的标本和切片中未发现AYV的颗粒，而PYFV的颗粒为等轴体，直径约30 nm。阴性染色。由几个至几百个直径约20至28 nm的病毒样颗粒组成的组。电镜下观察到C. AYV/PYFV复合物的蚜虫中，但不是在健康植物或PYFV单独来源上喂养的蚜虫中。颗粒被密集染色材料包围，然后嵌入轻度染色材料（M材料）基质中，覆盖咽部腹侧壁（前肠前部）内膜的15 - 20 µ m长部分。在该区域，内膜增厚并形成几个尖峰状突起。M物质也发生在以健康植物为食的蚜虫中，但比以受感染植物为食的蚜虫少。在一些蚜虫，固定后立即喂养AYV感染的植物，其他组的病毒样颗粒被发现游离在前肠腔。在矩阵中的颗粒没有发现，直到蚜虫喂AYV感染的植物足够长的时间来获得可传播的病毒，但他们仍然存在于蚜虫喂了2小时后，10%的蔗糖喂了24小时的AYV源。在蜕皮的蚜虫，基质，与相关的病毒样颗粒，仍然附着在旧的咽内衬。据认为，病毒样颗粒是AYV的颗粒，基质是蚜虫内的特定滞留部位。在携带AYV的蚜虫和携带AYV和PYFV的蚜虫之间没有观察到差异，并且不知道在携带两种病毒的昆虫中发现的一些颗粒是否是PYFV的颗粒。
The aphidCavariella aegopodiitransmits anthriscus yellows virus (AYV) and parsnip yellow fleck virus (PYFV) in a semi-persistent manner and loses them on moulting. It transmits PYFV only when it is also carrying AYV. The particles of AYV have not been seen in preparations or sections from infected plants but those of PYFV are isometric, about 30 nm in diam. in negative stain. Groups of a few to several hundred virus-like particles about 20 to 28 nm in diam. were seen by electron microscopy in the foregut ofC. aegopodiitransmitting AYV or the AYV/PYFV complex but not in aphids fed on healthy plants or on a source of PYFV alone. The particles were surrounded by densely staining material, which was in turn embedded in a matrix of lightly staining material (M-material), overlying a 15 to 20 µm-long portion of the intima lining the ventral wall of the pharynx (anterior portion of the foregut). In this region the intima is thickened and formed into several spike-like protrusions. The M-material also occurred in aphids fed on healthy plants, but less commonly than in those fed on infected plants. In some aphids that were fixed immediately after feeding on AYV-infected plants, additional groups of virus-like particles were found free in the foregut lumen. The particles in the matrix were not found until the aphids had fed on AYV-infected plants long enough to acquire transmissible virus, but they were still present in aphids that had fed for 2 h on 10% sucrose after feeding for 24 h on a source of AYV. In moulting aphids, the matrix, with associated virus-like particles, remained attached to the old pharynx lining. It is thought that the virus-like particles are those of AYV and that the matrix is the specific site of retention within the aphid. No differences were observed between aphids carrying AYV and those carrying both AYV and PYFV, and it is not known whether some of the particles found in insects carrying both viruses are those of PYFV.