Fractone aging in the subventricular zone of the lateral ventricle
Fractone aging in the subventricular zone of the lateral ventricle
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DOI：
10.1016/j.jchemneu.2015.06.001
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发表时间：
2015-07-01
影响因子：
2.8
通讯作者：
Arikawa-Hirasawa, Eri
中科院分区：
文献类型：
--
作者：
Kerever, Aurelien;Yamada, Taihei;Arikawa-Hirasawa, Eri
成年后，脑室下区(SVZ)是神经发生持续存在的受限区域之一。在这个神经性的小生境中，专门的细胞外基质(ECM)结构被称为断裂，联系神经干细胞及其直系后代。破碎酮是由无处不在的细胞外基质成分组成的，包括硫酸乙酰肝素蛋白多糖，如perlecan和agrin。我们之前已经证明，裂解酮可以捕获生长因子，并通过肝素结合机制促进生长因子活性，从而调节神经发生。众所周知，随着年龄的增长，神经发生会减少。然而，老化对分形体结构和组成的影响尚不清楚。在这里，我们报告，虽然分音数目减少，但随着年龄的增长，分音的大小显著增加。尽管碎裂带形态发生了变化，但表达胶质纤维酸性蛋白的壁龛细胞与裂隙带保持着直接接触。此外，我们还观察到，随着时间的推移，破碎酮中所含的硫酸乙酰肝素链发生了修饰。然而，成纤维细胞生长因子-2仍然被通过硫酸乙酰肝素的碎裂酮捕获。总之，我们的结果表明，所观察到的分形酮结构和组成的变化与SVZ神经源性生态位的老化密切相关。(C)2015爱思唯尔B.V.保留所有权利。
In adulthood, the subventricular zone (SVZ) is one of the restricted places where neurogenesis persists. In this neurogenic niche, specialized extracellular matrix (ECM) structures termed fractones contact neural stem cells and their immediate progeny. Fractones are composed of ubiquitous ECM components including heparan sulfate proteoglycans such as perlecan and agrin. We have previously shown that fractones can capture growth factors and promote growth factor activity through a heparin binding mechanism in order to regulate neurogenesis. With aging, neurogenesis is known to decrease. However, the effect of aging on fractones structure and composition remains unknown. Here, we report that, while fractone number decreased, fractone size dramatically increased with aging. Despite the changes in fractones morphology, niche cells expressing glial fibrillary acidic protein kept direct contact with fractones. Furthermore, we have observed that heparan sulfate chains contained in fractones were modified with aging. However, FGF-2 was still captured by fractones via heparan sulfates. Together, our results suggest that the changes observed in fractones structure and composition are critically related to aging of the SVZ neurogenic niche. (C) 2015 Elsevier B.V. All rights reserved.