Hindlimb movement in the cat induced by amplitude-modulated stimulation using extra-spinal electrodes.
Hindlimb movement in the cat induced by amplitude-modulated stimulation using extra-spinal electrodes.
复制标题
使用脊髓外电极进行调幅刺激引起猫的后肢运动。
DOI：
10.1088/1741-2560/5/2/002
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发表时间：
2008
影响因子：
4
通讯作者：
deGroat,WilliamC
中科院分区：
文献类型：
--
作者：
Tai,Changfeng;Wang,Jicheng;Shen,Bing;Wang,Xianchun;Roppolo,JamesR;deGroat,WilliamC
本实验观察了猫在T13-L1水平急性脊髓横断前后，用L5-S1脊髓背侧表面或L5-S1背/腹根调幅波形电刺激诱发的后肢运动。在同一脊髓节段刺激脊髓或背/腹根可以引起相似的运动，包括协调的多关节屈曲或伸展。当刺激从肩部(L5)移至尾部(S1)节段时，诱发运动由屈曲变为伸展。刺激一侧背根或腹根仅引起同侧后肢运动。然而，沿中线或跨脊髓对脊髓背侧表面的刺激可引起双侧运动。刺激L7背根引起的伸展产生的地面反作用力最大，足以支撑体重。刺激背根引起的地面反作用力比刺激腹根引起的地面反作用力大，并产生更渐变的募集曲线。通过在不同刺激通道之间选择适当的时机，联合刺激肩部(L5)和尾部(L6/L7)脊髓节段，可以产生不同速度的步态。急性横断脊髓并没有改变反应，这表明诱发的运动不需要脊柱上运动中心的参与。本研究提出的方法和刺激策略可用于恢复脊髓损伤后的运动功能。
Hindlimb movement in the cat induced by electrical stimulation with an amplitude-modulated waveform of the dorsal surface of the L5-S1 spinal cord or the L5-S1 dorsal/ventral roots was investigated before and after acute spinal cord transection at the T13-L1 level. Stimulation of the spinal cord or dorsal/ventral root at the same spinal segment induced similar movements including coordinated multi-joint flexion or extension. The induced movements changed from flexion to extension when the stimulation was moved from rostral (L5) to caudal (S1) spinal segments. Stimulation of a dorsal or ventral root on one side induced only ipsilateral hindlimb movement. However, stimulation on the dorsal surface of the spinal cord along the midline or across the spinal cord induced bilateral movements. The extension induced by stimulation of L7 dorsal root produced the largest ground reaction force that was strong enough to support body weight. Dorsal root stimulation induced a larger ground reaction force than ventral root stimulation and produced a more graded recruitment curve. Stepping at different speeds could be generated by combined stimulation of the rostral (L5) and the caudal (L6/L7) spinal segments with an appropriate timing between the different stimulation channels. Acute transection of the spinal cord did not change the responses indicating that the induced movements did not require the involvement of the supraspinal locomotor centers. The methods and the stimulation strategy developed in this study might be utilized to restore locomotor function after spinal cord injury.