Dual characterization of biological cells by optofluidic microscope and resistive pulse sensor
Dual characterization of biological cells by optofluidic microscope and resistive pulse sensor
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DOI：
10.1002/elps.201400268
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发表时间：
2015-02-01
期刊：
影响因子：
2.9
通讯作者：
Kang, Yuejun
中科院分区：
文献类型：
--
作者：
Guo, Jinhong;Chen, Liang;Kang, Yuejun
无标记检测技术由于可以避免繁琐的样品前处理过程而成为生物医学应用的一个有力平台。在本文中，我们展示了一个双分析的生物细胞使用一个单一的微流控系统相结合的光流体显微镜和电阻脉冲传感。红细胞（RBC）和循环肿瘤细胞（CTC）都已被用于验证双重分析的概念，并且还用于测试微流体装置的性能。通过电阻脉冲感测的细胞表征与通过光流体显微镜的分析良好一致，进一步通过商业Beckman-Coulter（R）FC 500流式细胞术验证。该系统具有制作简单、易于集成、可移植性强、成本低等优点。本研究对于开发具有同时成像传感和电阻传感的创新性片上流式细胞术具有很大的潜力。
Label-free detection technique has emerged as a powerful platform for biomedical applications since it can avoid laborious multi-step sample preparation. In this paper, we demonstrate a dual analysis of biological cells using a single microfluidic system combining optofluidic microscopy and resistive pulse sensing. Both red blood cells (RBCs) and circulating tumor cells (CTCs) have been used to validate the concept of dual analysis and also to test the performance of the microfluidic device. The cell characterization by resistive pulse sensing is in good agreement with the analysis by optofluidic microscopy, further verified by the commercial Beckman-Coulter (R) FC500 flow cytometry. The present system has attractive merits such as simple fabrication, easy integration, high portability, and low cost. This study has great potentials for the development of innovative on-chip flow cytometry with concurrent imaging sensing and resistive sensing.