G proteins mediate changes in cell shape by stabilizing the axis of polarity
G proteins mediate changes in cell shape by stabilizing the axis of polarity
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DOI：
10.1016/s1097-2765(00)80325-1
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发表时间：
2000-05-01
期刊：
影响因子：
16
通讯作者：
Arkowitz, RA
中科院分区：
文献类型：
--
作者：
Nern, A;Arkowitz, RA
一旦接触到交配信息素，酵母细胞就会改变它们的形状，变成梨形的shmoos。我们研究了在交配过程中无法定向生长和无法选择芽位的突变体中依赖于信息素的细胞形状变化。在这些双重突变体中，细胞表面生长、分泌位点、细胞骨架和信息素受体都是分散的，这解释了为什么这些细胞是圆形的。相反，极性建立蛋白定位于这些突变体中的离散位点。然而，这些网站的位置徘徊。因此，这些突变体能够启动极化生长，但不能保持生长位点的位置。我们的研究结果表明，稳定的生长轴需要从信息素受体或特定的芽位点选择蛋白的位置信号。
Upon exposure to mating pheromone, yeast cells change their form to pear-shaped shmoos. We looked at pheromone-dependent cell shape changes in mutants that are unable to orient growth during mating and unable to choose a bud site. In these double mutants, cell surface growth, secretion sites, cytoskeleton, and pheromone receptors are spread out, explaining why these cells are round. In contrast, polarity establishment proteins localize to discrete sites in these mutants. However, the location of these sites wanders. Thus, these mutants are able to initiate polarized growth but fail to maintain the location of growth sites. Our results demonstrate that stabilization of the growth axis requires positional signaling from either the pheromone receptor or specific bud site selection proteins.