Thermodynamic modeling of donor splice site recognition in pre-mRNA.
Thermodynamic modeling of donor splice site recognition in pre-mRNA.
复制标题
前体 mRNA 中供体剪接位点识别的热力学模型。
DOI：
10.1103/physreve.69.041903
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Aalberts,DanielP
中科院分区：
文献类型：
--
作者：
Garland,JeffreyA;Aalberts,DanielP
当真核基因被剪接体编辑时，内含子识别的第一步是将U1小核RNA与供体剪接位点结合。我们建立了这种相互作用的热力学模型，以确定剪接位点。应用于一组65个带注释的基因，我们的“结合发现”方法实现了真实位点和假位点的显著分离。分析结合模式使我们能够丢弃大量的诱饵位点。我们的研究结果改进了基于统计的供体位点识别方法，展示了物理建模在基因组中寻找功能元素的前景。
When eukaryotic genes are edited by the spliceosome, the first step in intron recognition is the binding of a U1 small nuclear RNA with the donorsplice site. We model this interaction thermodynamically to identify splice sites. Applied to a set of 65 annotated genes, our “finding with binding” method achieves a significant separation between real and false sites. Analyzing binding patterns allows us to discard a large number of decoy sites. Our results improve statistics-based methods for donor site recognition, demonstrating the promise of physical modeling to find functional elements in the genome.