Specificity of action of colony-stimulating factors in the differentiation of granulocytes and macrophages.
Specificity of action of colony-stimulating factors in the differentiation of granulocytes and macrophages.
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DOI：
10.1002/9780470720998.ch2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
N. Nicola;D. Metcalf
中科院分区：
文献类型：
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作者：
N. Nicola;D. Metcalf
四种集落刺激因子（csf） （M-CSF、GM-CSF、Multi-CSF和G-CSF）均能刺激小鼠骨髓或胎儿肝脏祖细胞产生巨噬细胞。然而，它们对巨噬细胞祖细胞的相对选择性和刺激巨噬细胞祖细胞相对于其他祖细胞的剂量反应特性不同。目前尚不清楚是否存在对一种或另一种CSF具有独特反应性的不同祖细胞亚群，或者由不同CSF产生的巨噬细胞是否在功能上都是相同的。然而，本文显示，各种csf可以从母细胞祖细胞产生一种中间巨噬细胞祖细胞，其生长被凝集素刺激的脾细胞条件培养基中的一种物质特异性抑制。研究还表明，对于至少一种髓单细胞白血病细胞系，G-CSF诱导分化为巨噬细胞和粒细胞最有效，而M-CSF或Multi-CSF则不行。最后，研究了巨噬细胞和巨噬细胞系在诱导这些csf产生中的作用，以及它们对不同csf的特异性受体的显示。
Four colony-stimulating factors (CSFs) (M-CSF, GM-CSF, Multi-CSF and G-CSF) can each stimulate the production of macrophages from progenitor cells in murine bone marrow or fetal liver. However, they differ in their relative selectivity for macrophage progenitor cells and in their dose-response characteristics for stimulating macrophage progenitors relative to other progenitors. It is unresolved whether distinct subsets of progenitor cells exist with a unique responsiveness to one or other CSF or whether the macrophages produced by different CSFs are all functionally equivalent. However, it is shown here that various CSFs can generate from blast progenitor cells an intermediate macrophage progenitor cell whose growth is specifically inhibited by a substance in lectin-stimulated spleen cell-conditioned media. It is also shown that, for at least one myelomonocytic leukaemic cell line, differentiation to macrophages and granulocytes can be induced most effectively by G-CSF but not by M-CSF or Multi-CSF. Finally, the involvement of macrophages and macrophage cell lines in the induced production of these CSFs as well as their display of specific receptors for the different CSFs is examined.