Using synthetic peptide reagents to distinguish infections caused by different HIV strains.
Using synthetic peptide reagents to distinguish infections caused by different HIV strains.
复制标题
使用合成肽试剂区分不同HIV毒株引起的感染。
DOI：
10.1007/978-3-642-75267-4_8
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发表时间：
1990
影响因子：
--
通讯作者：
Oldstone,MB
中科院分区：
文献类型：
--
作者：
GnannJr,JW;Oldstone,MB
随着人类免疫缺陷病毒（HIV）其他分离株的核酸序列的确定，这组病毒中显著的异质性已经变得明显。艾滋病毒已被细分为艾滋病毒1型和艾滋病毒2型，前者是首先被证明会引起获得性免疫缺陷综合征（艾滋病）的逆转录病毒，后者是最近发现的一种流行于西非的逆转录病毒。HIV-1和HIV-2在相对保守的核心蛋白中约59%的位置和包膜蛋白中约42%的位置具有相同的氨基酸（Guyaderet al. 1987）。这两种病毒的核心蛋白之间存在部分抗原交叉反应性，但包膜蛋白之间的交叉反应性尚未得到描述（Clavelet al. 1986, 1987;Brun-Vezinetet al. 1987）。因此，含有HIV-1全病毒裂解物作为抗原的酶联免疫吸附试验（ELISA）可以检测到一些HIV-2阳性血清，但对于HIV-2的诊断来说，这是一个不一致和不可靠的测试。
As the nucleic acid sequences of additional isolates of human immunodeficiency virus (HIV) have been determined, the remarkable heterogeneity within this group of viruses has become apparent. HIV has been subclassified into HIV type 1, the retrovirus first shown to cause the acquired immunodeficiency syndrome (AIDS), and HIV type 2, a more recently discovered retrovirus prevalent in West Africa. HIV-1 and HIV-2 have identical amino acids at about 59% of positions in the relatively conserved core proteins and at about 42% of positions in the envelope proteins (Guyaderet al. 1987). There is partial antigenic cross-reactivity between the core proteins of the two viruses, but cross-reactivity between envelope proteins has not been described (Clavelet al. 1986, 1987;Brun-Vezinetet al. 1987). Consequently, an enzyme-linked immunosorbent assay (ELISA) containing HIV-1 whole-virus lysate as an antigen detects some HIV-2-positive sera, but is an inconsistent and unreliable test for HIV-2 diagnosis.