Altered patterns of the interferon-inducible gene IFI16 expression in head and neck squamous cell carcinoma: immunohistochemical study including correlation with retinoblastoma protein, human papillomavirus infection and proliferation index
Altered patterns of the interferon-inducible gene IFI16 expression in head and neck squamous cell carcinoma: immunohistochemical study including correlation with retinoblastoma protein, human papillomavirus infection and proliferation index
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DOI：
10.1111/j.1365-2559.2004.02000.x
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发表时间：
2004-12-01
期刊：
影响因子：
6.4
通讯作者：
Gariglio, M
中科院分区：
文献类型：
--
作者：
Azzimonti, B;Pagano, M;Gariglio, M
目的：为了研究干扰素（IFN）诱导基因IFI 16的表达是否与体内增殖活性呈负相关，我们比较了一系列头颈部鳞状细胞癌（HNSCCs）中IFI 16的免疫组化反应性及其增殖指数和细胞周期调节因子pRb。由于人乳头瘤病毒（HPV）感染表现为宿主基因，如IFN-诱导基因的功能或表达水平的变化，我们还调查了HPV DNA的存在，以确定是否可以区分与HPV DNA相关的头颈部癌肿瘤，大概是由其他mechanism.Methods转化：36 HNSCCs进行了评估IFI 16，pRb和Ki 67表达免疫组化。HPV的存在也通过聚合酶链反应检测。9个肿瘤位于口咽部（扁桃体区）和27在喉。结果：发现在14个25（56%）喉鳞状细胞癌和5个9（56%）扁桃体鳞状细胞癌标本检查的HPV DNA; 17个19例HPV-DNA阳性的情况下，表现出高级别的IFI 16表达。总体而言，增殖活性与肿瘤分化和组织学分级显著相关。IFI 16蛋白表达与Ki 67呈负相关（P = 0.039）。低增殖肿瘤阳性的IFI 16染色表现出显着的表达pRb和更好的预后比那些肿瘤有低的IFI 16，pRb水平和高增殖index.Conclusions：据我们所知，这是第一次在HNSCC的IFN诱导的IFI 16基因的表达分析。低增殖肿瘤的阳性表达的IFI 16染色显示了显着的pRb和较好的预后比那些肿瘤有低的IFI 16，pRb水平和高增殖指数。
Aims: To investigate whether the expression of interferon (IFN)-inducible gene IFI16 is inversely related to proliferative activity in vivo, we compared immunohistochemical reactivity of IFI16 in a series of head and neck squamous cell carcinomas (HNSCCs) with their proliferation index and the cell cycle regulator pRb. As human papillomavirus (HPV) infection is manifested by changes in the function or expression level of host genes such as IFN-inducible genes, we also investigated the presence of HPV DNA to determine whether head and neck cancers associated with HPV DNA can be distinguished from tumours that are presumably transformed by other mechanisms.Methods: Thirty-six HNSCCs were evaluated for IFI16, pRb and Ki67 expression by immunohistochemistry. The presence of HPV was also detected by polymerase chain reaction. Nine tumours were located in the oropharynx (tonsillar area) and 27 in the larynx.Results: HPV DNA was found in 14 of 25 (56%) laryngeal SCCs and in five of nine (56%) tonsillar SCC specimens examined; 17 out of the 19 HPV-DNA-positive cases showed high-grade IFI16 expression. Overall, proliferative activity was significantly related to tumour differentiation and histological grading. IFI16 protein expression was significantly inversely correlated with Ki67 (P = 0.039). Low-proliferating tumours positive for IFI16 staining showed a marked expression of pRb and a better prognosis than those whose tumours had low IFI16, pRb levels and a high proliferation index.Conclusions: To our knowledge, this is the first expression analysis of the IFN-inducible IFI16 gene in HNSCC. Low-proliferating tumours positive for IFI16 staining showed a marked expression of pRb and a better prognosis than those whose tumours had low IFI16, pRb levels and a high proliferation index.