Characterization, prevalence and antibiogram study of Staphylococcus aureus in poultry
Characterization, prevalence and antibiogram study of Staphylococcus aureus in poultry
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DOI：
10.1016/j.apjtb.2016.12.001
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发表时间：
2017-03-01
影响因子：
1.7
通讯作者：
Marzan, Lolo Wal
中科院分区：
文献类型：
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作者：
Ali, Yeasmeen;Islam, Md. Ashraful;Marzan, Lolo Wal
目的：了解耐甲氧西林金黄色葡萄球菌（S。方法：对来自孟加拉国吉大港市不同家禽养殖场的样品进行MRSA检测，并对5种抗生素的耐药谱进行分析。金黄色葡萄球菌通过不同的生化试验，并通过使用PCR鉴定mecA基因的存在来确认MRSA。沙门氏菌的耐药模式。结果：共采集60份标本（鼻拭子30份，泄殖腔拭子30份），其中54份经鉴定为金黄色葡萄球菌。金黄色葡萄球菌通过不同的生化试验。其中12例经PCR检测mecA基因证实为MRSA。在革兰氏染色研究中，鼻腔和泄殖腔样本对青霉素G的耐药性最高，对新霉素的耐药性最低。结论：根据目前的研究，可以说，不同的抗生素被广泛使用在家禽中，导致MRSA，是人类健康的警报。
Objective: To reveal the presence of methicillin resistant Staphylococcus aureus (S. aureus) (MRSA) in poultry samples and to determine the antibiogram pattern against five antibiotics.Methods: Samples from different poultry farm of Chittagong city, Bangladesh were examined for S. aureus by different biochemical tests and confirmed as MRSA by identifying the presence of mecA gene using PCR. Antibiotic resistance pattern in S. aureus was determined by antibiotic disk diffusion method.Results: In this study, a total of 60 samples (30 from nasal swabs and 30 from cloacal swabs) were used, of which 54 were confirmed as S. aureus by different biochemical tests. Among these, 12 were confirmed as MRSA by detecting mecA gene using PCR. During antibiogram study, both nasal and cloacal samples showed the highest resistance against penicillin-G and the lowest resistance was observed against neomycin.Conclusions: Based on the present study, it can be said that different antibiotics are used extensively in poultry that leads to MRSA and is alarming for human health.