The use of metabolomics integrated with transcriptomic and proteomic studies for identifying key steps involved in the control of nitrogen metabolism in crops such as maize
The use of metabolomics integrated with transcriptomic and proteomic studies for identifying key steps involved in the control of nitrogen metabolism in crops such as maize
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DOI：
10.1093/jxb/ers186
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发表时间：
2012-09-01
影响因子：
6.9
通讯作者：
Hirel, Bertrand
中科院分区：
文献类型：
--
作者：
Amiour, Nardjis;Imbaud, Sandrine;Hirel, Bertrand
将植物表型与基因和蛋白质表达以及代谢物合成和积累联系起来是改善全球农业生产的主要挑战之一。这种挑战与作物氮素利用效率（NUE）特别相关。在这里，叶基因转录，蛋白质和代谢产物积累在玉米长期氮（N）缺乏的生长条件下，在植物发育的两个重要阶段的差异进行了研究。氮缺乏的影响进行了检查，在转录组学，蛋白质组学和代谢组学水平。研究发现，当氮受到限制时，许多关键的植物生物学功能被上调或下调，包括光合作用、碳（C）代谢的重大改变，以及在较小程度上下游代谢途径的改变。研究还发现，就转录物、蛋白质和代谢物积累而言，缺氮胁迫的影响类似于植物对许多其他生物和非生物胁迫的反应。氮素同化和灌浆期的遗传和代谢变化是不同的，表明植物发育是一个重要组成部分，以确定参与控制植物氮素利用率的关键因素。还发现，整合三个卵组学的研究并不简单，因为从基因表达到代谢物积累，不同水平的调控似乎以逐步的方式发生。本文讨论了这些组学研究的潜在用途，以期提高我们对全株氮经济学的理解，这在育种和农学上应具有应用价值。
Linking plant phenotype to gene and protein expression and also to metabolite synthesis and accumulation is one of the main challenges for improving agricultural production worldwide. Such a challenge is particularly relevant to crop nitrogen use efficiency (NUE). Here, the differences in leaf gene transcript, protein, and metabolite accumulation in maize subjected to long-term nitrogen (N)-deficient growth conditions at two important stages of plant development have been studied. The impact of N deficiency was examined at the transcriptomic, proteomic, and metabolomic levels. It was found that a number of key plant biological functions were either up- or down-regulated when N was limiting, including major alterations to photosynthesis, carbon (C) metabolism, and, to a lesser extent, downstream metabolic pathways. It was also found that the impact of the N deficiency stress resembled the response of plants to a number of other biotic and abiotic stresses, in terms of transcript, protein, and metabolite accumulation. The genetic and metabolic alterations were different during the N assimilation and the grain-filling period, indicating that plant development is an important component for identifying the key elements involved in the control of plant NUE. It was also found that integration of the three oomics' studies is not straightforward, since different levels of regulation seem to occur in a stepwise manner from gene expression to metabolite accumulation. The potential use of these oomics' studies is discussed with a view to improve our understanding of whole plant nitrogen economics, which should have applications in breeding and agronomy.