Pathogenesis of a virus-induced leukemia in mice.
Pathogenesis of a virus-induced leukemia in mice.
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病毒诱导的小鼠白血病的发病机制。
DOI：
10.1093/jnci/26.1.189
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发表时间：
1961
期刊：
影响因子：
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通讯作者：
B. Arnold
中科院分区：
文献类型：
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作者：
T. Dunn;J. Moloney;A. W. Green;B. Arnold
将接受病毒诱导白血病小鼠脾脏无细胞提取物的雄性BALB/c小鼠与接受正常脾脏无细胞提取物的相同年龄和性别的BALB/c小鼠进行比较。未处理的BALB/c小鼠用作对照。选择6周龄的小鼠进行注射，因为新生小鼠的组织难以处理，并且给予相对低剂量的病毒以确保白血病的逐渐发展。直到8周后，在病毒注射小鼠的骨髓中观察到许多含有粒细胞的巨核细胞时，才能检测到三组之间的差异。检测到的下一个差异是注射病毒的小鼠脾脏增生逐渐增加。少数小鼠淋巴结中出现局部网状细胞增生。最显著的变化似乎是在胸腺中，除了一个例外，在这个器官中，白血病首先在治疗的小鼠中被识别出来。一只白血病小鼠的胸腺不含恶性细胞，但显示肉芽肿反应。由原发性白血病制成的一系列移植导致在生长期、从接种部位扩散和器官受累方面具有不同生物学特性的淋巴细胞肿瘤。从没有白血病形态学证据的小鼠的增生脾脏转移组织，再现了与转移无细胞提取物后观察到的相同的白血病前病变序列。本研究表明，接种白血病诱导病毒导致小鼠某些网状器官的紊乱，这有利于淋巴细胞肿瘤的发展。莫洛尼描述的病毒可能像许多其他致癌物质一样起作用。几个偶然的病变出现在实验，对照，和股票BALB/c小鼠的描述。
Male BALB/c mice that received a cell-free extract from the spleens of mice with leukemia induced by a virus were compared with BALB/c mice of the same age and sex that received a cell-free extract from normal spleens. Untreated BALB/c mice were used as controls. Mice 6 weeks of age were chosen for the injections, since tissues from newborn mice are hard to handle, and a relatively low dose of virus was given to insure a gradual development of leukemia. No difference in the three groups could be detected until 8 weeks had elapsed, when many megakaryocytes containing granulocytes were observed in the bone marrow of the virus-injected mice. The next difference detected was a gradually increasing hyperplasia of the spleen in the virus-injected mice. A few mice showed a localized reticulum-cell hyperplasia in the lymph nodes. The most significant alterations appeared to be in the thymus, and, with one exception, it was in this organ that leukemia was first recognized in the treated mice. The thymus in one leukemic mouse did not contain malignant cells but showed a granulomatous reaction. A series of transplants made from the primary leukemias resulted in lymphocytic neoplasms with different biologic characteristics in regard to growth period, spread from the site of inoculation, and organ involvement. The transfer of tissue from hyperplastic spleens from mice with no morphologic evidence of leukemia reproduced the same sequence of preleukemic lesions that were observed after transfer of the cell-free extract. This study indicates that inoculation of the leukemia-inducing virus causes a derangement in certain reticular organs of mice which is conducive to the development of lymphocytic neoplasms. The virus described by Moloney may be acting like many other carcinogenic agents. Several incidental lesions appearing in experimental, control, and stock BALB/c mice are described.