The Effects of the Surface Properties of a Gold Nanorod on Its In Vitro/Vivo Toxicity Against Cancer Cells
The Effects of the Surface Properties of a Gold Nanorod on Its In Vitro/Vivo Toxicity Against Cancer Cells
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金纳米棒的表面特性对其体外/体内癌细胞毒性的影响
DOI：
10.1166/jbn.2019.2851
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发表时间：
2019-11-01
影响因子：
2.9
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Gong, Jing;Zhang, Yingying;Li, Wei
金纳米棒（GNR）对癌细胞表现出细胞毒性。同时，它对非肿瘤细胞几乎没有影响。 GNR与亚细胞细胞器之间相互作用的理解对于确定细胞内机制起着非常重要的作用。我们所做的目的是解释 GNR 的表面特性对特定癌细胞死亡的影响。通过种子介导的生长方法精细制备了三种不同纵横比的 GNR 样品。然后通过透射电子显微镜（TEM）、共焦激光扫描显微镜（CLSM）、激光散射和流式细胞术（FCM）研究癌细胞死亡的细胞内运输和体外3种体内机制。结果发现，GNRs700表现出最大的光热转换效率。然而，有或没有光刺激的GNR660对癌细胞表现出最高的细胞毒性，这与一般知识相矛盾。详细的细胞内研究表明，溶酶体是影响 GNR 功能的关键亚细胞器。进一步的实验表明，细胞毒性受到 GNR 表面电位的强烈影响。这种电位实际上与表面阳离子分子的密度有关，从而进一步调节溶酶体膜的渗透。本文获得的结果表明表面电位的物理化学性质介导了 GNR 针对肿瘤的特异性毒性。
Gold nano rods (GNRs) have showed cytotoxicity to cancer cells. At the same time, it shows little effects on non-tumor cells. Between GNRs and sub-cellular organelles, the understanding of interaction plays a very important role to determine the intracellular mechanisms. The purpose of what we done is to explain the effects of the surface properties of GNRs on specific cancer cell death. Three GNR samples with different aspect ratios were finely prepared by the seed-mediated growth method. Then the intracellular transport and the in vitro 3 vivo mechanisms of cancer cell death were studied by transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), laser light scattering, and flow cytometry (FCM). It was found that GNRs700 exhibited the largest photothermal conversion efficiency. However, the GNR660 with or without light stimulation exhibited the highest cytotoxicity against cancer cells, which was contradict to the general knowledge. Detailed intracellular investigations showed that the lysosome was the key sub-organelle affecting the GNR function. Further experiments revealed that cytotoxicity was strongly affected by the GNR's surface potential. This potential was actually related to the density of surface cationic molecules, which further regulated lysosomal membrane penetration. The results obtained herein indicated that the physicochemical properties of the surface potential mediated the specific toxicity of GNRs against tumours.